Identification of Histoplasma capsulatum from culture extracts by real-time PCR

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organis...

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Published in:Journal of clinical microbiology Vol. 41; no. 3; p. 1295
Main Authors: Martagon-Villamil, Jose, Shrestha, Nabin, Sholtis, Mary, Isada, Carlos M, Hall, Gerri S, Bryne, Terry, Lodge, Barbara A, Reller, L Barth, Procop, Gary W
Format: Journal Article
Language:English
Published: United States 01.03.2003
Subjects:
DNA, Fungal - analysis
Histoplasma - genetics
Histoplasma - isolation & purification
Humans
Microbiological Techniques
Polymerase Chain Reaction - methods
ISSN:0095-1137
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Abstract We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
AbstractList We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
Author Bryne, Terry
Reller, L Barth
Martagon-Villamil, Jose
Hall, Gerri S
Sholtis, Mary
Isada, Carlos M
Shrestha, Nabin
Procop, Gary W
Lodge, Barbara A
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  surname: Martagon-Villamil
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  givenname: Nabin
  surname: Shrestha
  fullname: Shrestha, Nabin
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  givenname: Carlos M
  surname: Isada
  fullname: Isada, Carlos M
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  givenname: Gerri S
  surname: Hall
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  surname: Bryne
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  surname: Reller
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  givenname: Gary W
  surname: Procop
  fullname: Procop, Gary W
BackLink https://www.ncbi.nlm.nih.gov/pubmed/12624071$$D View this record in MEDLINE/PubMed
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Snippet We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107...
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SubjectTerms DNA, Fungal - analysis
Histoplasma - genetics
Histoplasma - isolation & purification
Humans
Microbiological Techniques
Polymerase Chain Reaction - methods
Title Identification of Histoplasma capsulatum from culture extracts by real-time PCR
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