Identification of Histoplasma capsulatum from culture extracts by real-time PCR

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organis...

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Podrobná bibliografia
Vydané v:	Journal of clinical microbiology Ročník 41; číslo 3; s. 1295
Hlavní autori:	Martagon-Villamil, Jose, Shrestha, Nabin, Sholtis, Mary, Isada, Carlos M, Hall, Gerri S, Bryne, Terry, Lodge, Barbara A, Reller, L Barth, Procop, Gary W
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 01.03.2003
Predmet:	DNA, Fungal - analysis Histoplasma - genetics Histoplasma - isolation & purification Humans Microbiological Techniques Polymerase Chain Reaction - methods
ISSN:	0095-1137
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Popis
Shrnutí:	We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0095-1137
DOI:	10.1128/JCM.41.3.1295-1298.2003