Cooperative control of blood compatibility and re-endothelialization by immobilized heparin and substrate topography

[Display omitted] A wide variety of environmental cues provided by the extracellular matrix, including biophysical and biochemical cues, are responsible for vascular cell behavior and function. In particular, substrate topography and surface chemistry have been shown to regulate blood and vascular c...

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Vydáno v:	Acta biomaterialia Ročník 15; s. 150 - 163
Hlavní autoři:	Ding, Yonghui, Yang, Meng, Yang, Zhilu, Luo, Rifang, Lu, Xiong, Huang, Nan, Huang, Pingbo, Leng, Yang
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England Elsevier Ltd 01.03.2015
Témata:	Adhesion Biocompatibility Blood Blood compatibility Blood Platelets - cytology Blood Platelets - drug effects Blood Platelets - ultrastructure Cell Adhesion - drug effects Cell Shape - drug effects Cell Survival - drug effects Coculture Techniques Cues Determinants Elongation Endothelial cell Endothelial cells Focal Adhesions - drug effects Focal Adhesions - metabolism Heparin Heparin - pharmacology Heparins Human Umbilical Vein Endothelial Cells - drug effects Human Umbilical Vein Endothelial Cells - physiology Humans Materials Testing Partial Thromboplastin Time Phenotype Photoelectron Spectroscopy Platelet Adhesiveness - drug effects Stress Fibers - drug effects Substrate topography Surface Properties Topography Blood compatibility Endothelial cell Heparin Substrate topography
ISSN:	1742-7061, 1878-7568, 1878-7568
On-line přístup:	Získat plný text
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Shrnutí:	[Display omitted] A wide variety of environmental cues provided by the extracellular matrix, including biophysical and biochemical cues, are responsible for vascular cell behavior and function. In particular, substrate topography and surface chemistry have been shown to regulate blood and vascular compatibility individually. The combined impact of chemical and topographic cues on blood and vascular compatibility, and the interplay between these two types of cues, are subjects that are currently being explored. In the present study, a facile polydopamine-mediated approach is introduced for immobilization of heparin on topographically patterned substrates, and the combined effects of these cues on blood compatibility and re-endothelialization are systematically investigated. The results show that immobilized heparin and substrate topography cooperatively modulate anti-coagulation activity, endothelial cell (EC) attachment, proliferation, focal adhesion formation and endothelial marker expression. Meanwhile, the substrate topography is the primary determinant of cell alignment and elongation, driving in vivo-like endothelial organization. Importantly, combining immobilized heparin with substrate topography empowers substantially greater competitive ability of ECs over smooth muscle cells than each cue individually. Moreover, a model is proposed to elucidate the cooperative interplay between immobilized heparin and substrate topography in regulating cell behavior.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1742-7061 1878-7568 1878-7568
DOI:	10.1016/j.actbio.2014.12.014