A network model of the molecular organization of chromatin in Drosophila

Chromatin governs gene regulation and genome maintenance, yet a substantial fraction of the chromatin proteome is still unexplored. Moreover, a global model of the chromatin protein network is lacking. By screening >100 candidates we identify 42 Drosophila proteins that were not previously associ...

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Veröffentlicht in:Molecular cell Jg. 49; H. 4; S. 759
Hauptverfasser: van Bemmel, Joke G, Filion, Guillaume J, Rosado, Arantxa, Talhout, Wendy, de Haas, Marcel, van Welsem, Tibor, van Leeuwen, Fred, van Steensel, Bas
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 21.02.2013
Schlagworte:
Animals
Bayes Theorem
Binding Sites
Cell Line
Chromatin - metabolism
Chromosomes, Insect - metabolism
DNA Repair
DNA Replication
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Drosophila Proteins - physiology
Gene Expression Regulation
Models, Biological
Molecular Sequence Annotation
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Nuclear Proteins - physiology
Principal Component Analysis
Protein Binding
Protein Interaction Mapping
Protein Interaction Maps
Protein Processing, Post-Translational
ISSN:1097-4164, 1097-4164
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Zusammenfassung:Chromatin governs gene regulation and genome maintenance, yet a substantial fraction of the chromatin proteome is still unexplored. Moreover, a global model of the chromatin protein network is lacking. By screening >100 candidates we identify 42 Drosophila proteins that were not previously associated with chromatin, which all display specific genomic binding patterns. Bayesian network modeling of the binding profiles of these and 70 known chromatin components yields a detailed blueprint of the in vivo chromatin protein network. We demonstrate functional compartmentalization of this network, and predict functions for most of the previously unknown chromatin proteins, including roles in DNA replication and repair, and gene activation and repression.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1097-4164
1097-4164
DOI:10.1016/j.molcel.2013.01.040