Imaging linear and circular polarization features in leaves with complete Mueller matrix polarimetry

Spectropolarimetry of intact plant leaves allows to probe the molecular architecture of vegetation photosynthesis in a non-invasive and non-destructive way and, as such, can offer a wealth of physiological information. In addition to the molecular signals due to the photosynthetic machinery, the cel...

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Vydané v:Biochimica et biophysica acta. General subjects Ročník 1862; číslo 6; s. 1350 - 1363
Hlavní autori: Patty, C.H. Lucas, Luo, David A., Snik, Frans, Ariese, Freek, Buma, Wybren Jan, ten Kate, Inge Loes, van Spanning, Rob J.M., Sparks, William B., Germer, Thomas A., Garab, Győző, Kudenov, Michael W.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Netherlands Elsevier B.V 01.06.2018
Predmet:
absorbance
Algorithms
chlorophyll
Chlorophyll a
Chloroplast
chloroplasts
Circular dichroism
circular dichroism spectroscopy
corn
Image Processing, Computer-Assisted - methods
leaves
Light
Microscopy, Polarization - methods
Mueller matrix polarimetry
Photosynthesis
Plant Leaves - growth & development
Plant Leaves - metabolism
polarimetry
Refractometry - methods
vegetation
Zea mays - growth & development
Zea mays - metabolism
Mueller matrix polarimetry
Photosynthesis
Chloroplast
Circular dichroism
Chlorophyll a
ISSN:0304-4165, 1872-8006
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Shrnutí:Spectropolarimetry of intact plant leaves allows to probe the molecular architecture of vegetation photosynthesis in a non-invasive and non-destructive way and, as such, can offer a wealth of physiological information. In addition to the molecular signals due to the photosynthetic machinery, the cell structure and its arrangement within a leaf can create and modify polarization signals. Using Mueller matrix polarimetry with rotating retarder modulation, we have visualized spatial variations in polarization in transmission around the chlorophyll a absorbance band from 650 nm to 710 nm. We show linear and circular polarization measurements of maple leaves and cultivated maize leaves and discuss the corresponding Mueller matrices and the Mueller matrix decompositions, which show distinct features in diattenuation, polarizance, retardance and depolarization. Importantly, while normal leaf tissue shows a typical split signal with both a negative and a positive peak in the induced fractional circular polarization and circular dichroism, the signals close to the veins only display a negative band. The results are similar to the negative band as reported earlier for single macrodomains. We discuss the possible role of the chloroplast orientation around the veins as a cause of this phenomenon. Systematic artefacts are ruled out as three independent measurements by different instruments gave similar results. These results provide better insight into circular polarization measurements on whole leaves and options for vegetation remote sensing using circular polarization. •Complete imaging Mueller matrix polarimetry and decomposition on leaves around the chlorophyll absorbance band (650 nm–710 nm).•Distinctive spectropolarimetric features for the normal tissue and veins were distinguished in the Mueller matrix elements and decomposition.•Large differences in circular polarizance/dichroism: solely a negative signal in the veins versus the typical split signal in normal tissue.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0304-4165
1872-8006
DOI:10.1016/j.bbagen.2018.03.005