A second capsidless hadakavirus strain with 10 positive-sense single-stranded RNA genomic segments from Fusarium nygamai

A unique capsidless virus with a positive-sense, single-stranded RNA genome (hadakavirus 1, HadV1), a member of the extended picorna-like supergroup, was isolated previously from the phytopathogenic fungus Fusarium oxysporum . Here, we describe the molecular and biological characterisation of a seco...

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Vydáno v:Archives of virology Ročník 166; číslo 10; s. 2711 - 2722
Hlavní autoři: Khan, Haris Ahmed, Sato, Yukiyo, Kondo, Hideki, Jamal, Atif, Bhatti, Muhammad Faraz, Suzuki, Nobuhiro
Médium: Journal Article
Jazyk:angličtina
Vydáno: Vienna Springer Vienna 01.10.2021
Springer Nature B.V
Témata:
Asymptomatic infection
Biomedical and Life Sciences
Biomedicine
Double-stranded RNA
Fusarium
Fusarium oxysporum
genome
Genomes
Genomics
Infectious Diseases
Medical Microbiology
Nucleotide sequence
nucleotide sequences
Original Article
phenotype
Phylogenetics
Phytopathogenic fungi
plant pathogenic fungi
Proteins
Replicative form
ribonucleases
Ribonucleic acid
RNA
RNA polymerase
sequence analysis
Spores
Virology
Viruses
Zinc finger proteins
ISSN:0304-8608, 1432-8798, 1432-8798
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Shrnutí:A unique capsidless virus with a positive-sense, single-stranded RNA genome (hadakavirus 1, HadV1), a member of the extended picorna-like supergroup, was isolated previously from the phytopathogenic fungus Fusarium oxysporum . Here, we describe the molecular and biological characterisation of a second hadakavirus strain from Fusarium nygamai , which has not been investigated in detail previously as a virus host. This virus, hadakavirus 1 strain 1NL (HadV1-1NL), has features similar to the first hadakavirus, HadV1-7n, despite having a different number of segments (10 for HadV1-1NL vs. 11 for HadV1-7n). The 10 genomic RNA segments of HadV1-1NL range in size from 0.9 kb to 2.5 kb. All HadV1-1NL segments show 67% to 86% local nucleotide sequence identity to their HadV1-7n counterparts, whereas HadV1-1NL has no homolog of HadV1-7n RNA8, which encodes a zinc-finger motif. Another interesting feature is the possible coding incapability of HadV1-1NL RNA10. HadV1-1NL was predicted to be capsidless based on the RNase A susceptibility of its replicative form dsRNA. Phenotypic comparison of multiple virus-infected and virus-free single-spore isolates indicated asymptomatic infection by HadV1-1NL. Less-efficient vertical transmission via spores was observed as the infected fungal colonies from which the spores were derived became older, as was observed for HadV1-7n. This study shows a second example of a hadakavirus that appears to have unusual features.
Bibliografie:ObjectType-Article-1
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ISSN:0304-8608
1432-8798
1432-8798
DOI:10.1007/s00705-021-05176-x