Acute myocardial infarction activates distinct inflammation and proliferation pathways in circulating monocytes, prior to recruitment, and identified through conserved transcriptional responses in mice and humans

Monocytes play critical roles in tissue injury and repair following acute myocardial infarction (AMI). Specifically targeting inflammatory monocytes in experimental models leads to reduced infarct size and improved healing. However, data from humans are sparse, and it remains unclear whether monocyt...

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Veröffentlicht in:European heart journal Jg. 36; H. 29; S. 1923
Hauptverfasser: Ruparelia, Neil, Godec, Jernej, Lee, Regent, Chai, Joshua T, Dall'Armellina, Erica, McAndrew, Debra, Digby, Janet E, Forfar, J Colin, Prendergast, Bernard D, Kharbanda, Rajesh K, Banning, Adrian P, Neubauer, Stefan, Lygate, Craig A, Channon, Keith M, Haining, Nicholas W, Choudhury, Robin P
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England 01.08.2015
Schlagworte:
Aged
Animals
Case-Control Studies
Cell Proliferation - physiology
Female
Gene Expression Profiling
Humans
Inflammation - immunology
Inflammation - pathology
Leukocytes, Mononuclear - immunology
Leukocytes, Mononuclear - pathology
Ligation
Magnetic Resonance Angiography
Male
Mice, Inbred C57BL
Middle Aged
Myocardial Infarction - genetics
Myocardial Infarction - immunology
Myocardial Infarction - pathology
Phenotype
Transcription, Genetic - genetics
Transcription, Genetic - immunology
Transcriptional Activation - physiology
Monocytes
Inflammation
Acute myocardial infarction
Mitosis
Genomics
ISSN:1522-9645, 1522-9645
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Zusammenfassung:Monocytes play critical roles in tissue injury and repair following acute myocardial infarction (AMI). Specifically targeting inflammatory monocytes in experimental models leads to reduced infarct size and improved healing. However, data from humans are sparse, and it remains unclear whether monocytes play an equally important role in humans. The aim of this study was to investigate whether the monocyte response following AMI is conserved between humans and mice and interrogate patterns of gene expression to identify regulated functions. Thirty patients (AMI) and 24 control patients (stable coronary atherosclerosis) were enrolled. Female C57BL/6J mice (n = 6/group) underwent AMI by surgical coronary ligation. Myocardial injury was quantified by magnetic resonance imaging (human) and echocardiography (mice). Peripheral monocytes were isolated at presentation and at 48 h. RNA from separated monocytes was hybridized to Illumina beadchips. Acute myocardial infarction resulted in a significant peripheral monocytosis in both species that positively correlated with the extent of myocardial injury. Analysis of the monocyte transcriptome following AMI demonstrated significant conservation and identified inflammation and mitosis as central processes to this response. These findings were validated in both species. Our findings show that the monocyte transcriptome is conserved between mice and humans following AMI. Patterns of gene expression associated with inflammation and proliferation appear to be switched on prior to their infiltration of injured myocardium suggesting that the specific targeting of inflammatory and proliferative processes in these immune cells in humans are possible therapeutic strategies. Importantly, they could be effective in the hours after AMI.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1522-9645
1522-9645
DOI:10.1093/eurheartj/ehv195