Epidemiology and phylogenetic analysis of crimean-congo hemorrhagic fever viruses in xinjiang, china

In 2004 and 2005, an epidemiological survey of Crimean-Congo hemorrhagic fever virus (CCHFV) was conducted in Xinjiang, China. A total of 5,629 serum samples of human and livestock were collected and tested for the CCHFV antibody, and 17,319 ticks were collected for viral identification. Reverse pas...

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Vydané v:Journal of clinical microbiology Ročník 47; číslo 8; s. 2536
Hlavní autori: Sun, Surong, Dai, Xiang, Aishan, Muhetaer, Wang, Xinhui, Meng, Weiwei, Feng, Conghui, Zhang, Fuchun, Hang, Changshou, Hu, Zhihong, Zhang, Yujiang
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 01.08.2009
Predmet:
Animals
Animals, Domestic - immunology
Antibodies, Viral - blood
China - epidemiology
Cluster Analysis
Hemagglutination Inhibition Tests - methods
Hemorrhagic Fever Virus, Crimean-Congo - classification
Hemorrhagic Fever Virus, Crimean-Congo - genetics
Hemorrhagic Fever Virus, Crimean-Congo - isolation & purification
Hemorrhagic Fever, Crimean - epidemiology
Hemorrhagic Fever, Crimean - veterinary
Hemorrhagic Fever, Crimean - virology
Humans
Mice
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Sequence Homology
Seroepidemiologic Studies
Serum - immunology
Ticks - virology
China
ISSN:1098-660X, 1098-660X
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Shrnutí:In 2004 and 2005, an epidemiological survey of Crimean-Congo hemorrhagic fever virus (CCHFV) was conducted in Xinjiang, China. A total of 5,629 serum samples of human and livestock were collected and tested for the CCHFV antibody, and 17,319 ticks were collected for viral identification. Reverse passive hemagglutination inhibition assays showed that the average prevalence of CCHFV antibody was 1.7% for the humans and 12.7% for the livestock. A relatively high antibody prevalence, ranging from 19.1% to 23.4%, was found in the livestock of the northwest, southwest, and northeast parts of the Tarim Basin. When the ticks were pooled to inoculate suckling mice, followed by reverse transcription-PCR (RT-PCR) to detect CCHFV RNA, the average RT-PCR-positive rates for Hyalomma asiaticum kozlovi and H. asiaticum asiaticum were 12.9% and 2.6%, respectively. A significant correlation was found between the antibody prevalence in the livestock and the CCHFV prevalence in H. asiaticum of the same geographic region. No CCHFV RNA was detected in Dermacentor nivenus, Rhipicephalus turanius, or Rhipicephalus sanguineus. A total of 27 partial S segments of CCHFVs were sequenced and used for phylogeny analysis. All but one Chinese isolate grouped into the Asia 1 clade, which contains the strains from Xinjiang and Uzbekistan, while the other strain, Fub90009, grouped with strains from the Middle East.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1098-660X
1098-660X
DOI:10.1128/JCM.00265-09