The Bor1 elevator transport cycle is subject to autoinhibition and activation

Boron, essential for plant growth, necessitates precise regulation due to its potential toxicity. This regulation is achieved by borate transporters (BORs), which are homologous to the SLC4 family. The Arabidopsis thaliana Bor1 (AtBor1) transporter from clade I undergoes slow regulation through degr...

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Veröffentlicht in:Nature communications Jg. 15; H. 1; S. 9090 - 12
Hauptverfasser: Jiang, Yan, Jiang, Jiansen
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 22.10.2024
Nature Publishing Group
Nature Portfolio
Schlagworte:
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Anion Transport Proteins - genetics
Anion Transport Proteins - metabolism
Antiporters
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Biological Transport
Borates - chemistry
Borates - metabolism
Boron
Boron - metabolism
Cryoelectron Microscopy
Electron microscopy
Humanities and Social Sciences
Models, Molecular
multidisciplinary
Mutagenesis
Phosphorylation
Plant growth
Science
Science (multidisciplinary)
Toxicity
ISSN:2041-1723, 2041-1723
Online-Zugang:Volltext
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Beschreibung
Zusammenfassung:Boron, essential for plant growth, necessitates precise regulation due to its potential toxicity. This regulation is achieved by borate transporters (BORs), which are homologous to the SLC4 family. The Arabidopsis thaliana Bor1 (AtBor1) transporter from clade I undergoes slow regulation through degradation and translational suppression, but its potential for fast regulation via direct activity modulation was unclear. Here, we combine cryo-electron microscopy, mutagenesis, and functional characterization to study AtBor1, revealing high-resolution structures of the dimer in one inactive and three active states. Our findings show that AtBor1 is regulated by two distinct mechanisms: an autoinhibitory domain at the carboxyl terminus obstructs the substrate pathway via conserved salt bridges, and phosphorylation of Thr410 allows interaction with a positively charged pocket at the cytosolic face, essential for borate transport. These results elucidate the molecular basis of AtBor1’s activity regulation and highlight its role in fast boron level regulation in plants. Boron is essential for plant growth, and its transport is tightly regulated. Here, the authors present structures of the borate transporter AtBor1 in both inactive and active states, revealing how its C-terminal domain mediates autoinhibition.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-024-53411-1