Simple, low-cost, sensitive and label-free aptasensor for the detection of cardiac troponin I based on a gold nanoparticles modified titanium foil

This research demonstrated the electrochemical modification of low-cost titanium (Ti) metal substrate with gold nanoparticles (AuNPs) for the aptamer-based detection of cardiac troponin I (cTnI). AuNPs were deposited onto Ti sheets by the potential-step deposition method with high density and homoge...

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Veröffentlicht in:Biosensors & bioelectronics Jg. 126; S. 381 - 388
Hauptverfasser: Lopa, Nasrin Siraj, Rahman, Md. Mahbubur, Ahmed, Faiz, Ryu, Taewook, Sutradhar, Sabuj Chandra, Lei, Jin, Kim, Jaewoong, Kim, Dae Ho, Lee, Yong Hoon, Kim, Whangi
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Elsevier B.V 01.02.2019
Schlagworte:
Aptamer
aptasensors
blood serum
Cardiac troponin I
crystal structure
Differential pulse voltammetry
DNA
electrochemistry
foil
Gold nanoparticles
humans
Label-free
nanogold
oligonucleotides
Ti foil
titanium
troponin I
Aptamer
Cardiac troponin I
Ti foil
Label-free
Gold nanoparticles
Differential pulse voltammetry
ISSN:0956-5663, 1873-4235, 1873-4235
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Zusammenfassung:This research demonstrated the electrochemical modification of low-cost titanium (Ti) metal substrate with gold nanoparticles (AuNPs) for the aptamer-based detection of cardiac troponin I (cTnI). AuNPs were deposited onto Ti sheets by the potential-step deposition method with high density and homogeneity as well as good crystallinity. It was then applied as a transducer to immobilize a thiol-functionalized DNA aptamer via the self-assembled monolayer mechanism for the specific binding of cTnI. This was verified through electrochemical and morphological analyses. The aptasensor could detect cTnI in a linear range of 1–1100 pM with a detection limit of ca. 0.18 pM. The aptasensor showed high sensitivity and specificity to cTnI over other interfering compounds with good recoveries in the diluted human serum samples. [Display omitted] •AuNPs modified Ti foil is prepared for the aptamer-based detection of cTnI.•High-density AuNPs allows maximizing the immobilization of cTnI-binding aptamers.•The oxidation signal variation of [Fe(CN)6]3-/4- with [cTnI] enables to detect cTnI.•This simple and low-cost aptasensor shows good analytical performance.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0956-5663
1873-4235
1873-4235
DOI:10.1016/j.bios.2018.11.012