Discriminative power of Campylobacter phenotypic and genotypic typing methods

The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. Campylobacter jejuni (n=94) and Campylobacter coli (n=52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (...

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Vydáno v:	Journal of microbiological methods Ročník 125; s. 33 - 39
Hlavní autoři:	Duarte, Alexandra, Seliwiorstow, Tomasz, Miller, William G., De Zutter, Lieven, Uyttendaele, Mieke, Dierick, Katelijne, Botteldoorn, Nadine
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Netherlands Elsevier B.V 01.06.2016
Témata:	Animals antibiotic resistance Antimicrobial resistance Bacterial Typing Techniques - methods Bacterial Typing Techniques - standards Broiler meat Campylobacter coli Campylobacter coli - classification Campylobacter coli - genetics Campylobacter coli - isolation & purification Campylobacter jejuni Campylobacter jejuni - classification Campylobacter jejuni - genetics Campylobacter jejuni - isolation & purification chicken meat Drug Resistance, Multiple, Bacterial - genetics flaA-RFLP flagellin Flagellin - genetics Food Food Microbiology - methods genes Genotyping Techniques - methods Genotyping Techniques - standards lipooligosaccharides Lipopolysaccharides - classification Meat - microbiology meat carcasses MLST monitoring multilocus sequence typing Multilocus Sequence Typing - methods Phenotype Polymorphism, Restriction Fragment Length Poultry - microbiology restriction fragment length polymorphism Typing methods virulence Virulence Factors - genetics Typing methods MLST flaA-RFLP Campylobacter coli Antimicrobial resistance Broiler meat Campylobacter jejuni Food
ISSN:	0167-7012, 1872-8359
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Shrnutí:	The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. Campylobacter jejuni (n=94) and Campylobacter coli (n=52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (MLST), flagellin gene A restriction fragment length polymorphism typing (flaA-RFLP), antimicrobial resistance profiling (AMRp), the presence/absence of 5 putative virulence genes; and, exclusively for C. jejuni, the determination of lipooligosaccharide (LOS) class. Discriminatory power was calculated by the Simpson's index of diversity (SID) and the congruence was measured by the adjusted Rand index and adjusted Wallace coefficient. MLST was individually the most discriminative typing method for both C. jejuni (SID=0.981) and C. coli (SID=0.957). The most discriminative combination with a SID of 0.992 for both C. jejuni and C. coli was obtained by combining MLST with flaA-RFLP. The combination of MLST with flaA-RFLP is an easy and feasible typing method for short-term monitoring of Campylobacter in broiler meat carcass. •MLST/flaA-RFLP combination had the highest typing discrimination in this study.•Discriminatory power of combined methods relies on congruence between methods.•MLST/flaA-RFLP is feasible for short-term monitoring of broiler meat for Campylobacter.•MLST/flaA-RFLP and MLST/AMRp provided equivalent discriminatory power.
Bibliografie:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2016.03.004