Discriminative power of Campylobacter phenotypic and genotypic typing methods

The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. Campylobacter jejuni (n=94) and Campylobacter coli (n=52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (...

Full description

Saved in:
Bibliographic Details
Published in:Journal of microbiological methods Vol. 125; pp. 33 - 39
Main Authors: Duarte, Alexandra, Seliwiorstow, Tomasz, Miller, William G., De Zutter, Lieven, Uyttendaele, Mieke, Dierick, Katelijne, Botteldoorn, Nadine
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01.06.2016
Subjects:
Animals
antibiotic resistance
Antimicrobial resistance
Bacterial Typing Techniques - methods
Bacterial Typing Techniques - standards
Broiler meat
Campylobacter coli
Campylobacter coli - classification
Campylobacter coli - genetics
Campylobacter coli - isolation & purification
Campylobacter jejuni
Campylobacter jejuni - classification
Campylobacter jejuni - genetics
Campylobacter jejuni - isolation & purification
chicken meat
Drug Resistance, Multiple, Bacterial - genetics
flaA-RFLP
flagellin
Flagellin - genetics
Food
Food Microbiology - methods
genes
Genotyping Techniques - methods
Genotyping Techniques - standards
lipooligosaccharides
Lipopolysaccharides - classification
Meat - microbiology
meat carcasses
MLST
monitoring
multilocus sequence typing
Multilocus Sequence Typing - methods
Phenotype
Polymorphism, Restriction Fragment Length
Poultry - microbiology
restriction fragment length polymorphism
Typing methods
virulence
Virulence Factors - genetics
Typing methods
MLST
flaA-RFLP
Campylobacter coli
Antimicrobial resistance
Broiler meat
Campylobacter jejuni
Food
ISSN:0167-7012, 1872-8359
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. Campylobacter jejuni (n=94) and Campylobacter coli (n=52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (MLST), flagellin gene A restriction fragment length polymorphism typing (flaA-RFLP), antimicrobial resistance profiling (AMRp), the presence/absence of 5 putative virulence genes; and, exclusively for C. jejuni, the determination of lipooligosaccharide (LOS) class. Discriminatory power was calculated by the Simpson's index of diversity (SID) and the congruence was measured by the adjusted Rand index and adjusted Wallace coefficient. MLST was individually the most discriminative typing method for both C. jejuni (SID=0.981) and C. coli (SID=0.957). The most discriminative combination with a SID of 0.992 for both C. jejuni and C. coli was obtained by combining MLST with flaA-RFLP. The combination of MLST with flaA-RFLP is an easy and feasible typing method for short-term monitoring of Campylobacter in broiler meat carcass. •MLST/flaA-RFLP combination had the highest typing discrimination in this study.•Discriminatory power of combined methods relies on congruence between methods.•MLST/flaA-RFLP is feasible for short-term monitoring of broiler meat for Campylobacter.•MLST/flaA-RFLP and MLST/AMRp provided equivalent discriminatory power.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2016.03.004