Simultaneous determination of three diarylheptanoids and an α-tetralone derivative in the green walnut husks ( Juglans regia L.) by high-performance liquid chromatography with photodiode array detector

By optimizing extraction, separation and analytical conditions, a reliable and accurate high-performance liquid chromatographic (HPLC) method coupled with photodiode array detector (DAD) at room temperature is developed for simultaneous determination of three diarylheptanoids (juglanin A, juglanin B...

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Vydáno v:	Journal of Chromatography A Ročník 1190; číslo 1; s. 80 - 85
Hlavní autoři:	Liu, Junxi, Meng, Min, Li, Chen, Huang, Xinyi, Di, Duolong
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Amsterdam Elsevier B.V 09.05.2008 Amsterdam; New York: Elsevier Elsevier
Témata:	alpha-tetralone aromatic compounds Biological and medical sciences chemical structure Chromatography, High Pressure Liquid - methods Crystallography, X-Ray detectors Diarylheptanoids Diarylheptanoids - analysis Diarylheptanoids - chemistry General pharmacology high performance liquid chromatography HPLC hulls juglanin A juglanin B Juglans - chemistry Juglans regia Juglans regia L Medical sciences medicinal plants Molecular Structure Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments photodiode array detectors Reference Standards rhioptelol Spectrophotometry, Ultraviolet Tetralones - analysis Tetralones - chemistry walnuts α-Tetralone Asia China α-Tetralone Diarylheptanoids HPLC Juglans regia L Chemical analysis HPLC chromatography Solvent extraction Oxygen heterocycle Macrocycle Medicinal plant Sample preparation Folk medicine Dicotyledones Angiospermae Bicyclic compound Diode array Aromatic compound Fruit tree Cyclophane Husk (fruit) Quantitative analysis Juglans regia Validation Pharmacognosy Tricyclic compound Ketophenols Juglandaceae Simultaneous measurement Plant origin Spermatophyta Juglans regia L.,Diarylheptanoids,α-Tetralone,HPLC Ultraviolet visible spectrometry
ISSN:	0021-9673
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Popis
Shrnutí:	By optimizing extraction, separation and analytical conditions, a reliable and accurate high-performance liquid chromatographic (HPLC) method coupled with photodiode array detector (DAD) at room temperature is developed for simultaneous determination of three diarylheptanoids (juglanin A, juglanin B, rhoiptelol) and an α-tetralone derivative (regiolone) in methanol extracts from the green walnut husks ( Juglans regia L.) The sample pretreatment process involved the reflux extraction using methanol as the extract with a ratio of liquor to sample of 15 mL/g. The separation was achieved on a SinoChrom ODS-AP C 18 column with gradient elution using acetonitrile and 2% (v/v) acetic acid in water. The intra-day and inter-day precision (RSD%) for the analytes ranged from 1.08 to 1.51 and 0.60 to 1.13, respectively. The average recoveries obtained were from 88.4% to 96.2% for the analytes with RSDs below 3.13%. The correlation coefficients of the calibration curve exceeded 0.999. The detection limits were 0.51, 0.25, 0.32 and 0.35 ng at a signal-to-noise ratio of 3, respectively. Quantitative analyses of the samples from different grown sites and in obtained different months showed that the contents of the analytes varied significantly. The method was then successfully applied for the detection and isolation of a new diarylheptanoid derivative in the green walnut husks ( J. regia L.). The structure of the new compound was elucidated by various spectroscopic methods including 2D NMR techniques (COSY, HMQC, HMBC), HR-ESI-MS and X-ray single-crystal diffraction analysis.
Bibliografie:	http://dx.doi.org/10.1016/j.chroma.2008.02.083 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9673
DOI:	10.1016/j.chroma.2008.02.083