Immunohistochemical Localization of Collagen Types I, III, and IV, Laminin, Fibronectin, and Keratin in the Endolymphatic Sac

We have employed immunohistochemistry to obtain baseline information on the molecular constituents of the extracellular matrix (ECM) of the endolymphatic duct (ED) and endolymphatic sac (ES) of the chinchilla. The results demonstrated that collagen types I and III were distributed in the subepitheli...

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Bibliographic Details
Published in:Annals of Otology, Rhinology & Laryngology Vol. 109; no. 2; pp. 180 - 186
Main Authors: Harada, Teruhiko, Kim, Youngki, Juhn, Steven K., Sakakura, Yasuo
Format: Journal Article
Language:English
Published: Los Angeles, CA SAGE Publications 01.02.2000
Annals Publishing Compagny
SAGE PUBLICATIONS, INC
Subjects:
Animals
Biological and medical sciences
Chinchilla
Collagen
Collagen - analysis
Endolymphatic Duct
Endolymphatic Duct - chemistry
Endolymphatic Sac
Endolymphatic Sac - chemistry
Ent. Stomatology
Extracellular Matrix
Extracellular Matrix - chemistry
Fibronectins
Fibronectins - analysis
Immunohistochemistry
Investigative techniques, diagnostic techniques (general aspects)
Keratins
Keratins - analysis
Laminin
Laminin - analysis
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
keratin
immunohistochemistry
endolymphatic sac
extracellular matrix
Immunohistochemistry
Healthy subject
Rodentia
Exploration
Chinchilla
Fibronectin
Pathology
Keratin
Endolymphatic sac
Vertebrata
Regulation(control)
Type
Mammalia
Laminin
Animal
Collagen
Localization
ISSN:0003-4894, 1943-572X
Online Access:Get full text
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Summary:We have employed immunohistochemistry to obtain baseline information on the molecular constituents of the extracellular matrix (ECM) of the endolymphatic duct (ED) and endolymphatic sac (ES) of the chinchilla. The results demonstrated that collagen types I and III were distributed in the subepithelial layer in the ED and ES, type IV collagen and laminin in the basement membranes, and fibronectin in the subepithelial layer and partly in the conglomerated cells in the ES. Collagen type III was diffusely distributed in the whole subepithelial layer of the ES, whereas collagen type I was concentrated densely in the deep layer of the interstitium, although gradually, the cuboidal epithelium in the ES was transformed into a flatter type in the ED. The epithelial cells of the ED and ES were clearly positive for keratin. This study deals, in particular, with the normal distribution of ECM components of the ED and ES of the chinchilla.
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ISSN:0003-4894
1943-572X
DOI:10.1177/000348940010900213