MicroRNA

MicroRNAs (miRNAs) are small endogenous RNAs that regulate gene-expression posttranscriptionally. MiRNA research in allergy is expanding because miRNAs are crucial regulators of gene expression and promising candidates for biomarker development. MiRNA mimics and miRNA inhibitors currently in preclin...

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Veröffentlicht in:Journal of allergy and clinical immunology Jg. 141; H. 4; S. 1202 - 1207
Hauptverfasser: Lu, Thomas X., Rothenberg, Marc E.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Elsevier Inc 01.04.2018
Elsevier Limited
Schlagworte:
Acids
Adsorption
Allergies
Allergy
antagomiRs
antimiRs
Binding sites
Biomarkers
Body fluids
CRISPR
CRISPR/Cas9
Gene expression
Gene Expression Regulation
gene silencing
Genetic Markers
Humans
Hybridization
Hypersensitivity - drug therapy
Hypersensitivity - genetics
Hypersensitivity - metabolism
in situ hybridization
In Vitro Techniques
Methods
microarray
microRNA
microRNA methods
MicroRNAs
MicroRNAs - genetics
MicroRNAs - isolation & purification
MicroRNAs - metabolism
MicroRNAs - therapeutic use
miRNA
miRNA expression
miRNA inhibitors
miRNA isolation
miRNA mimics
miRNA profiling
miRNA quantitation
miRNA targets
Phenols
qPCR
Quantitation
stem-loop
miRNA mimics
stem-loop
Allergy
CRISPR
miRNA profiling
CRISPR/Cas9
gene silencing
microRNA methods
miRNA inhibitors
antagomiRs
miRNA quantitation
microarray
miRNA expression
UTR
antimiRs
miRNA targets
miRNA isolation
microRNA
miRNA
qPCR
Cas9
in situ hybridization
ISSN:0091-6749, 1097-6825, 1097-6825
Online-Zugang:Volltext
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Beschreibung
Zusammenfassung:MicroRNAs (miRNAs) are small endogenous RNAs that regulate gene-expression posttranscriptionally. MiRNA research in allergy is expanding because miRNAs are crucial regulators of gene expression and promising candidates for biomarker development. MiRNA mimics and miRNA inhibitors currently in preclinical development have shown promise as novel therapeutic agents. Multiple technological platforms have been developed for miRNA isolation, miRNA quantitation, miRNA profiling, miRNA target detection, and modulating miRNA levels in vitro and in vivo. Here we will review the major technological platforms with consideration given for the advantages and disadvantages of each platform.
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ISSN:0091-6749
1097-6825
1097-6825
DOI:10.1016/j.jaci.2017.08.034