Overexpression of NtWRKY50 Increases Resistance to Ralstonia solanacearum and Alters Salicylic Acid and Jasmonic Acid Production in Tobacco

WRKY transcription factors (TFs) modulate plant responses to biotic and abiotic stresses. Here, we characterized a WRKY IIc TF, NtWRKY50, isolated from tobacco ( ) plants. The results showed that NtWRKY50 is a nuclear-localized protein and that its gene transcript is induced in tobacco when inoculat...

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Veröffentlicht in:Frontiers in plant science Jg. 8; S. 1710
Hauptverfasser: Liu, Qiuping, Liu, Ying, Tang, Yuanman, Chen, Juanni, Ding, Wei
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Switzerland Frontiers Media SA 11.10.2017
Frontiers Media S.A
Schlagworte:
Acid production
Bacteria
Disease resistance
Gene expression
Gene silencing
Genes
Hormones
Hydrogen peroxide
Infections
Jasmonic acid
Localization
NtWRKY50
Pathogens
Phenotypes
Plant bacterial diseases
Plant diseases
Plant immunity
Plant resistance
Plant Science
Proteins
Ralstonia solanacearum
Salicylic acid
Signal transduction
Stresses
Tobacco
Transcription factors
Wounding
NtWRKY50
salicylic acid
Ralstonia solanacearum
disease resistance
jasmonic acid
ISSN:1664-462X, 1664-462X
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Zusammenfassung:WRKY transcription factors (TFs) modulate plant responses to biotic and abiotic stresses. Here, we characterized a WRKY IIc TF, NtWRKY50, isolated from tobacco ( ) plants. The results showed that NtWRKY50 is a nuclear-localized protein and that its gene transcript is induced in tobacco when inoculated with the pathogenic bacterium . Overexpression of enhanced bacterial resistance, which correlated with enhanced SA and JA/ET signaling genes. However, silencing of the gene had no obvious effects on plant disease resistance, implying functional redundancy of NtWRKY50 with other TFs. In addition, it was found that can be induced by various biotic or abiotic stresses, such as Potato virus Y, , hydrogen peroxide, heat, cold, and wounding as well as the hormones salicylic acid (SA), jasmonic acid (JA), and ethylene (ET). Importantly, additional analysis suggests that overexpression markedly promotes SA levels but prevents pathogen-induced JA production. These data indicate that overexpression leads to altered SA and JA content, increased expression of defense-related genes and enhanced plant resistance to These probably due to increased activity of endogenous gene or could be gain-of-function phenotypes by altering the profile of genes affected by .
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Edited by: Alice Guidot, UMR2594 Laboratoire des Interactions Plantes Microorganismes (LIPM), France
This article was submitted to Plant Microbe Interactions, a section of the journal Frontiers in Plant Science
Reviewed by: Yasmina Jaufeerally Fakim, University of Mauritius, Mauritius; Tiffany M. Lowe-Power, University of Wisconsin-Madison, United States
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2017.01710