Environmental DNA metabarcoding: Transforming how we survey animal and plant communities

The genomic revolution has fundamentally changed how we survey biodiversity on earth. High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA from diverse kinds of environmental samples (termed “environmental DNA” or “eDNA”). Coupling HTS with our ability to associate seq...

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Vydané v:	Molecular ecology Ročník 26; číslo 21; s. 5872 - 5895
Hlavní autori:	Deiner, Kristy, Bik, Holly M., Mächler, Elvira, Seymour, Mathew, Lacoursière‐Roussel, Anaïs, Altermatt, Florian, Creer, Simon, Bista, Iliana, Lodge, David M., Vere, Natasha, Pfrender, Michael E., Bernatchez, Louis
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	England Blackwell Publishing Ltd 01.11.2017
Predmet:	Animals Biodiversity bioinformatic pipeline bioinformatics Biomonitoring citizen science conservation Conservation of Natural Resources Coupling (molecular) data collection Deoxyribonucleic acid DNA DNA barcoding DNA Barcoding, Taxonomic - methods DNA Primers DNA sequencing ecology Ecology - methods ecosystems eDNA education Environmental DNA Environmental Monitoring experimental design Filtration freshwater high-throughput nucleotide sequencing invasive species macro‐organism Nucleotide sequence Plant communities Plants Primers Relative abundance species diversity Species richness Surveying surveys Terrestrial environments Workflow bioinformatic pipeline conservation ecology species richness biomonitoring macro-organism citizen science eDNA invasive species
ISSN:	0962-1083, 1365-294X, 1365-294X
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Shrnutí:	The genomic revolution has fundamentally changed how we survey biodiversity on earth. High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA from diverse kinds of environmental samples (termed “environmental DNA” or “eDNA”). Coupling HTS with our ability to associate sequences from eDNA with a taxonomic name is called “eDNA metabarcoding” and offers a powerful molecular tool capable of noninvasively surveying species richness from many ecosystems. Here, we review the use of eDNA metabarcoding for surveying animal and plant richness, and the challenges in using eDNA approaches to estimate relative abundance. We highlight eDNA applications in freshwater, marine and terrestrial environments, and in this broad context, we distill what is known about the ability of different eDNA sample types to approximate richness in space and across time. We provide guiding questions for study design and discuss the eDNA metabarcoding workflow with a focus on primers and library preparation methods. We additionally discuss important criteria for consideration of bioinformatic filtering of data sets, with recommendations for increasing transparency. Finally, looking to the future, we discuss emerging applications of eDNA metabarcoding in ecology, conservation, invasion biology, biomonitoring, and how eDNA metabarcoding can empower citizen science and biodiversity education.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Review-3 content type line 23
ISSN:	0962-1083 1365-294X 1365-294X
DOI:	10.1111/mec.14350