Amide proton transfer (APT) contrast for imaging of brain tumors

In this work we demonstrate that specific MR image contrast can be produced in the water signal that reflects endogenous cellular protein and peptide content in intracranial rat 9L gliosarcomas. Although the concentration of these mobile proteins and peptides is only in the millimolar range, a detec...

Full description

Saved in:
Bibliographic Details
Published in:Magnetic resonance in medicine Vol. 50; no. 6; pp. 1120 - 1126
Main Authors: Zhou, Jinyuan, Lal, Bachchu, Wilson, David A., Laterra, John, van Zijl, Peter C.M.
Format: Journal Article
Language:English
Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.12.2003
Williams & Wilkins
Subjects:
9L gliosarcoma
amide proton exchange
Amides - analysis
Animals
Biological and medical sciences
Brain Neoplasms - chemistry
Brain Neoplasms - diagnosis
Cell Line, Tumor
Diffusion Magnetic Resonance Imaging
Gliosarcoma - chemistry
Gliosarcoma - diagnosis
Magnetic Resonance Imaging - methods
magnetization transfer
Medical sciences
mobile protein
Neoplasm Transplantation
peptide
Protons
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Rats
Rats, Inbred F344
Sensitivity and Specificity
Technology. Biomaterials. Equipments. Material. Instrumentation
magnetization transfer
peptide
9L gliosarcoma
mobile protein
amide proton exchange
Biomedical engineering
ISSN:0740-3194, 1522-2594
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this work we demonstrate that specific MR image contrast can be produced in the water signal that reflects endogenous cellular protein and peptide content in intracranial rat 9L gliosarcomas. Although the concentration of these mobile proteins and peptides is only in the millimolar range, a detection sensitivity of several percent on the water signal (molar concentration) was achieved. This was accomplished with detection sensitivity enhancement by selective radiofrequency (RF) labeling of the amide protons, and by utilizing the effective transfer of this label to water via hydrogen exchange. Brain tumors were also assessed by conventional T1‐weighted, T2‐weighted, and diffusion‐weighted imaging. Whereas these commonly‐used approaches yielded heterogeneous images, the new amide proton transfer (APT) technique showed a single well‐defined region of hyperintensity that was assigned to brain tumor tissue. Magn Reson Med 50:1120–1126, 2003. © 2003 Wiley‐Liss, Inc.
Bibliography:Whitaker Foundation
ark:/67375/WNG-Z6Q7SCT2-6
istex:D6FD5FE59B19FF87CEA58B7FDB7F45F66FC97DD1
NIH/NIBIB - No. EB02666
ArticleID:MRM10651
NIH/NINDS - No. NS31490
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0740-3194
1522-2594
DOI:10.1002/mrm.10651