In vitro Cell Migration and Invasion Assays

Migration is a key property of live cells and critical for normal development, immune response, and disease processes such as cancer metastasis and inflammation. Methods to examine cell migration are very useful and important for a wide range of biomedical research such as cancer biology, immunology...

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Vydáno v:Journal of visualized experiments číslo 88
Hlavní autoři: Justus, Calvin R., Leffler, Nancy, Ruiz-Echevarria, Maria, Yang, Li V.
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States MyJove Corporation 01.06.2014
Témata:
Animals
Bioengineering
biomedical research
cell culture
cell movement
Cell Movement - physiology
Chemotaxis - physiology
Cytological Techniques - instrumentation
Cytological Techniques - methods
immune response
inflammation
Melanoma, Experimental - pathology
metastasis
Mice
microscopy
neoplasm cells
Neoplasm Invasiveness
neoplasms
NIH 3T3 Cells
ISSN:1940-087X, 1940-087X
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Shrnutí:Migration is a key property of live cells and critical for normal development, immune response, and disease processes such as cancer metastasis and inflammation. Methods to examine cell migration are very useful and important for a wide range of biomedical research such as cancer biology, immunology, vascular biology, cell biology and developmental biology. Here we use tumor cell migration and invasion as an example and describe two related assays to illustrate the commonly used, easily accessible methods to measure these processes. The first method is the cell culture wound closure assay in which a scratch is generated on a confluent cell monolayer. The speed of wound closure and cell migration can be quantified by taking snapshot pictures with a regular inverted microscope at several time intervals. More detailed cell migratory behavior can be documented using the time-lapse microscopy system. The second method described in this paper is the transwell cell migration and invasion assay that measures the capacity of cell motility and invasiveness toward a chemo-attractant gradient. It is our goal to describe these methods in a highly accessible manner so that the procedures can be successfully performed in research laboratories even just with basic cell biology setup.
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Correspondence to: Li V. Yang at YANGL@ecu.edu
ISSN:1940-087X
1940-087X
DOI:10.3791/51046