Effect of KnockOut serum replacement on germ cell development of immature testis tissue culture

To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague–Dawley rats were cultured for 4 weeks in culture media supplemented with FBS or KSR. Tissue area was measured at the beginning and end of the culturing period....

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Vydané v:Theriogenology Ročník 85; číslo 2; s. 193 - 199
Hlavní autori: Liu, Feng, Cai, Chunhong, Wu, Xin, Cheng, Yanxia, Lin, Tao, Wei, Guanghui, He, Dawei
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States Elsevier Inc 15.01.2016
Predmet:
Animals
Apoptosis
Cattle
Culture Media
Culture medium
Fetal Blood
fetal bovine serum
Gene Expression
genetic markers
histology
Male
Rat
Rats
Rats, Sprague-Dawley
reverse transcriptase polymerase chain reaction
seminiferous tubules
Seminiferous Tubules - anatomy & histology
Serum
Serum replacement
spermatids
Spermatids - cytology
spermatocytes
Spermatocytes - cytology
Spermatogenesis
Spermatogenesis - genetics
spermatogonia
Spermatogonia - cytology
Spermatozoa - growth & development
staining
Testis
Testis - cytology
Testis - growth & development
Tissue culture
Tissue Culture Techniques
tissues
Culture medium
Testis
Tissue culture
Rat
Serum replacement
Spermatogenesis
ISSN:0093-691X, 1879-3231, 1879-3231
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Popis
Shrnutí:To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague–Dawley rats were cultured for 4 weeks in culture media supplemented with FBS or KSR. Tissue area was measured at the beginning and end of the culturing period. Testicular histology, development of the germ cells, and the diameter of seminiferous tubules were analyzed by hematoxylin and eosin staining. After 4 weeks in culture, apoptosis and expression of the stage-specific spermatogenesis marker genes Kit, Sycp3, and Crisp1 were assayed. Tissues cultured in KSR-supplemented media were able to sustain growth and gradually increase seminiferous tubule diameter during the culture period. In addition, spermatogonia, primary spermatocytes, secondary spermatocytes, and round spermatids were observed after 4 weeks in culture, and reverse transcription-PCR confirmed expression of the marker genes. In comparison, tissues cultured in FBS-supplemented media showed dwindling testicular organization, necrotic seminiferous tubules, and expression of Kit, but inconsistent expression of Sycp3 and Crisp1 KnockOut serum replacement outperforms FBS as a growth media supplements for culturing immature spermatogonial tissue culture.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0093-691X
1879-3231
1879-3231
DOI:10.1016/j.theriogenology.2015.09.012