MIF from mussel: Coding sequence, phylogeny, polymorphism, 3D model and regulation of expression

► Identification of MIF from Mytilus galloprovincialis. ► Phylogenetic relationships and design of the putative 3D structure. ► Sequence of numbers of variants from France and Sicily populations. ► Challenges resulted in general down regulation from 1 to 9–48h. Three macrophage migration inhibitory...

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Veröffentlicht in:	Developmental and comparative immunology Jg. 36; H. 4; S. 688 - 696
Hauptverfasser:	Parisi, Maria-Giovanna, Toubiana, Mylène, Mangano, Valentina, Parrinello, Nicolò, Cammarata, Matteo, Roch, Philippe
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Elsevier Ltd 01.04.2012
Schlagworte:	Amino Acid Sequence amino acid sequences amino acids animal models Animals Bacteria Base Sequence Biomphalaria glabrata Bivalve Candida albicans Challenge Chlamys Chlamys farreri Cloning, Molecular consensus sequence Cytokine Expressed Sequence Tags France Fungus gene expression Gene Expression Profiling gene expression regulation Gene Library Gene regulation Haliotis Hemocytes humans Innate immunity Italy Ixodidae Macrophage macrophage migration inhibitory factors Macrophage Migration-Inhibitory Factors - chemistry Macrophage Migration-Inhibitory Factors - genetics Macrophage Migration-Inhibitory Factors - immunology microarray technology Models, Molecular Molecular Sequence Data molecular weight Mollusk mussels mutation Mytilus Mytilus - genetics Mytilus - immunology Mytilus - metabolism Mytilus - microbiology Mytilus galloprovincialis nucleotides open reading frames Phylogeny Pinctada fucata q-PCR Sequence Alignment ticks yeasts France Italy Italy, Sicilia, Palermo ORF Mollusk Gene regulation Cytokine Mytilus EST Innate immunity q-PCR Challenge Fungus Mg-MIF UTR Bacteria Bivalve Macrophage
ISSN:	0145-305X, 1879-0089, 1879-0089
Online-Zugang:	Volltext
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Zusammenfassung:	► Identification of MIF from Mytilus galloprovincialis. ► Phylogenetic relationships and design of the putative 3D structure. ► Sequence of numbers of variants from France and Sicily populations. ► Challenges resulted in general down regulation from 1 to 9–48h. Three macrophage migration inhibitory factor (MIF)-related sequences were identified from a Mytilus galloprovincialis EST library. The consensus sequence included a 5′-UTR of 32 nucleotides, the complete ORF of 345 nucleotides, and a 3′-UTR of 349 nucleotides. As for other MIFs, M. galloprovincialis ORF does not include any signal or C-terminus extensions. The translated sequence of 115 amino acids possesses a molecular mass of 12,681.4, a pI of 6.27 and a stability index of 21.48. Its 3D structure resembles human MIF except for one shorter α-helix. Although evolutionary separated from ticks and vertebrates, Mg-MIF appeared to be closely related to Pinctada fucata and Haliotis, but not to Chlamys farreri and Biomphalaria glabrata. Numerous mutation points were observed within the Mg-MIF ORF, defining 11 amino acid variants within the mussels from Palavas-France and 14 amino acid variants within the mussels from Palermo-Italy. The 2 major variants from Palavas were identical to 2 of the 4 major variants from Palermo. In all the 18 Mg-MIF variants, residues involved in tautomerase and in oxidoreductase activities were conserved. Generally, one mussel expressed 2 Mg-MIF amino acid sequences but with different frequencies of occurrence. Mg-MIF is constitutively expressed principally in hemocytes and in the mantle. In contrast to other animal models, Mg-MIF expression was always down regulated following challenge by bacteria and fungi, confirming previous data obtained with microarray. Down regulation started as soon as 1h and Mg-MIF expression returned to background 9–48h after the challenge. Exception was regarding the yeast, Candidaalbicans, down-regulation between 9 and 72h, suggesting yeast and bacteria-filamentous fungi trigger different mechanisms of elimination.
Bibliographie:	http://dx.doi.org/10.1016/j.dci.2011.10.014 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0145-305X 1879-0089 1879-0089
DOI:	10.1016/j.dci.2011.10.014