XSP10 and SlSAMT, Fusarium wilt disease responsive genes of tomato (Solanum lycopersicum L.) express tissue specifically and interact with each other at cytoplasm in vivo

Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici ( Fol ) is a major fungal disease of tomato ( Solanum lycopersicum L.). Xylem sap protein 10 ( XSP10) and Salicylic acid methyl transferase ( SlSAMT) have been identified as putative negative regulatory genes associated with Fusarium wilt...

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Vydané v:Physiology and molecular biology of plants Ročník 27; číslo 7; s. 1559 - 1575
Hlavní autori: Debbarma, Johni, Saikia, Banashree, Singha, Dhanawantari L., Maharana, Jitendra, Velmuruagan, Natarajan, Dekaboruah, Hariprasanna, Arunkumar, Kallare P., Chikkaputtaiah, Channakeshavaiah
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: New Delhi Springer India 01.07.2021
Springer Nature B.V
Predmet:
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
biotic stress
Cell Biology
Cloning
Complementation
Cultivars
Cytoplasm
disease resistance
disease susceptibility
Disease tolerance
flowers
Fluorescence
Fungal diseases
fungi
Fusarium
Fusarium oxysporum
Fusarium oxysporum f. sp. lycopersici
Fusarium wilt
Gene expression
Genes
Genomics
Life Sciences
Localization
molecular biology
Plant Physiology
Plant Sciences
protein-protein interactions
Proteins
Protoplasts
quantitative polymerase chain reaction
Research Article
Salicylic acid
sap
Seedlings
Solanum lycopersicum
Tomatoes
Wilt
Xylem
Nucleus
In vivo
Fusarium wilt
XSP10
SlSAMT
Cytoplasm
ISSN:0971-5894, 0974-0430
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Shrnutí:Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici ( Fol ) is a major fungal disease of tomato ( Solanum lycopersicum L.). Xylem sap protein 10 ( XSP10) and Salicylic acid methyl transferase ( SlSAMT) have been identified as putative negative regulatory genes associated with Fusarium wilt of tomato. Despite their importance as potential genes for developing Fusarium wilt disease tolerance, very little knowledge is available about their expression, cell biology, and functional genomics. Semi-quantitative and quantitative real-time PCR expression analysis of XSP10 and SlSAMT, in this study, revealed higher expression in root and flower tissue respectively in different tomato cultivars viz. Micro-Tom (MT), Arka Vikas (AV), and Arka Abhed (AA). Therefore, the highly up-regulated expression of XSP10 and SlSAMT in biotic stress susceptible tomato cultivar (AV) than a multiple disease resistant cultivar (AA) suggested the disease susceptibility nature of these genes for Fusarium wilt. Sub-cellular localization analysis through the expression of gateway cloning constructs in tomato protoplasts and seedlings showed the predominant localization of XSP10 in the nucleus and SlSAMT at the cytoplasm. A strong in vivo protein–protein interaction of XSP10 with SlSAMT at cytoplasm from bi-molecular fluorescent complementation study suggested that these two proteins function together in regulating responses to Fusarium wilt tolerance in tomato.
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ISSN:0971-5894
0974-0430
DOI:10.1007/s12298-021-01025-y