Mycobacterial DNA extraction for whole-genome sequencing from early positive liquid (MGIT) cultures

We developed a low-cost and reliable method of DNA extraction from as little as 1 ml of early positive mycobacterial growth indicator tube (MGIT) cultures that is suitable for whole-genome sequencing to identify mycobacterial species and predict antibiotic resistance in clinical samples. The DNA ext...

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Bibliographic Details

Published in:	Journal of clinical microbiology Vol. 53; no. 4; p. 1137
Main Authors:	Votintseva, Antonina A, Pankhurst, Louise J, Anson, Luke W, Morgan, Marcus R, Gascoyne-Binzi, Deborah, Walker, Timothy M, Quan, T Phuong, Wyllie, David H, Del Ojo Elias, Carlos, Wilcox, Mark, Walker, A Sarah, Peto, Tim E A, Crook, Derrick W
Format:	Journal Article
Language:	English
Published:	United States 01.04.2015
Subjects:	Bacteriological Techniques - methods DNA, Bacterial - analysis DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Genome, Bacterial - genetics Humans Molecular Typing - methods Mycobacterium tuberculosis - genetics Sequence Analysis, DNA - methods Tuberculosis - diagnosis Tuberculosis - microbiology
ISSN:	1098-660X, 1098-660X
Online Access:	Get more information
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