MicroRNA-24 promotes 3T3-L1 adipocyte differentiation by directly targeting the MAPK7 signaling

Over the past years, MicroRNAs (miRNAs) act as a vital role in harmony with gene regulation and maintaining cellular homeostasis. It is well testified that miRNAshave been involved in numerous physiological and pathological processes, including embryogenesis, cell fate decision, and cellular differe...

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Published in:Biochemical and biophysical research communications Vol. 474; no. 1; pp. 76 - 82
Main Authors: Jin, Min, Wu, Yutao, Wang, Jing, Chen, Jian, Huang, Yiting, Rao, Jinpeng, Feng, Chun
Format: Journal Article
Language:English
Published: United States Elsevier Inc 20.05.2016
Subjects:
3T3-L1 adipocyte
3T3-L1 Cells
60 APPLIED LIFE SCIENCES
adipocytes
Adipocytes - cytology
Adipocytes - physiology
adipogenesis
Adipogenesis - physiology
Animals
binding proteins
CELL CYCLE
Cell Differentiation - physiology
CELL PROLIFERATION
Cell Proliferation - physiology
Differentiation
embryogenesis
Gene Expression Regulation, Developmental - physiology
GENE REGULATION
genes
HOMEOSTASIS
MAPK7
Mice
microRNA
MicroRNA-24
MicroRNAs - metabolism
mitogen-activated protein kinase
Mitogen-Activated Protein Kinase 7 - metabolism
peroxisome proliferator-activated receptors
RECEPTORS
signal transduction
SIGNALS
STEROLS
TRANSCRIPTION
TRANSCRIPTION FACTORS
triacylglycerols
TRIGLYCERIDES
MAPK7
Differentiation
MicroRNA-24
3T3-L1 adipocyte
ISSN:0006-291X, 1090-2104
Online Access:Get full text
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Summary:Over the past years, MicroRNAs (miRNAs) act as a vital role in harmony with gene regulation and maintaining cellular homeostasis. It is well testified that miRNAshave been involved in numerous physiological and pathological processes, including embryogenesis, cell fate decision, and cellular differentiation. Adipogenesis is an organized process of cellular differentiation by which pre-adipocytes differentiate towards mature adipocytes, and it is tightly modulated by a series of transcription factors such as peroxisome proliferator-activated receptor γ (PPAR-γ) and sterol regulatory-element binding proteins 1 (SREBP1). However, the molecular mechanisms underlying the connection between miRNAs and adipogenesis-related transcription factors remain obscure. In this study, we unveiled that miR- 24 was remarkably upregulated during 3T3-L1 adipogenesis. Overexpression of miR-24 significantly promoted 3T3-L1 adipogenesis, as evidenced by its ability to increase the expression of PPAR-γ and SREBP1, lipid droplet formation and triglyceride (TG) accumulation. Furthermore, we found that neither ectopic expression of miR-24nor miR-24 inhibitor affect cell proliferation and cell cycle progression. Finally, we demonstrated that miR-24 plays the modulational role by directly repressing MAPK7, a key number in the MAPK signaling pathway. These data indicate that miR-24 is a novel positive regulator of adipocyte differentiation by targeting MAPK7, which provides new insights into the molecular mechanism of miRNA-mediated cellular differentiation. •We firstly found miR-24 was upregulated in 3T3-L1 pre-adipocytes differentiation.•miR-24 promoted 3T3-L1 pre-adipocytes differentiation while silencing the expression of miR-24 had an opposite function.•miR-24 regulated 3T3-L1 differentiation by directly targeting MAPK7 signaling pathway.•miR-24did not affect 3T3-L1 pre-adipocytes cellular proliferation.
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ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2016.04.073