Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells

The Yin Yang 1 (YY1) transcription factor is a master regulator of development, essential for early embryogenesis and adult tissues formation. YY1 is the mammalian orthologue of Pleiohomeotic, one of the transcription factors that binds Polycomb DNA response elements in Drosophila melanogaster and m...

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Published in:Nucleic acids research Vol. 40; no. 8; pp. 3403 - 3418
Main Authors: Vella, Pietro, Barozzi, Iros, Cuomo, Alessandro, Bonaldi, Tiziana, Pasini, Diego
Format: Journal Article
Language:English
Published: England Oxford University Press 01.04.2012
Subjects:
Binding Sites
Cell Line
Drosophila melanogaster
E2F1 Transcription Factor - analysis
Embryonic Stem Cells - metabolism
Gene Expression Regulation
Gene Regulation, Chromatin and Epigenetics
Gene Regulatory Networks
Kruppel-Like Transcription Factors - metabolism
Polycomb-Group Proteins
Proto-Oncogene Proteins c-myc - metabolism
Repressor Proteins - analysis
RNA, Small Untranslated - metabolism
YY1 Transcription Factor - analysis
YY1 Transcription Factor - metabolism
ISSN:0305-1048, 1362-4962, 1362-4962
Online Access:Get full text
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Summary:The Yin Yang 1 (YY1) transcription factor is a master regulator of development, essential for early embryogenesis and adult tissues formation. YY1 is the mammalian orthologue of Pleiohomeotic, one of the transcription factors that binds Polycomb DNA response elements in Drosophila melanogaster and mediates Polycomb group proteins (PcG) recruitment to DNA. Despite several publications pointing at YY1 having a similar role in mammalians, others showed features of YY1 that are not compatible with PcG functions. Here, we show that, in mouse Embryonic Stem (ES) cells, YY1 has genome-wide PcG-independent activities while it is still stably associated with the INO80 chromatin-remodeling complex, as well as with novel RNA helicase activities. YY1 binds chromatin in close proximity of the transcription start site of highly expressed genes. Loss of YY1 functions preferentially led to a down-regulation of target genes expression, as well as to an up-regulation of several small non-coding RNAs, suggesting a role for YY1 in regulating small RNA biogenesis. Finally, we found that YY1 is a novel player of Myc-related transcription factors and that its coordinated binding at promoters potentiates gene expression, proposing YY1 as an active component of the Myc transcription network that links ES to cancer cells.
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ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkr1290