Asymmetrically lesioned mesencephalon in healthy rodents: call for caution

Stereological counting of tyrosine-hydroxylase immunoreactive (TH-IR) neurons in the mesencephalon is a pivotal parameter in assessing the extent of lesioning in animal models of Parkinson's disease. We here show that the number of TH-IR neurons often appears abnormally decreased in healthy—com...

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Vydáno v:	Brain research Ročník 1022; číslo 1; s. 251 - 253
Hlavní autoři:	Pioli, Elsa Y., Dovero, Sandra, Bioulac, Bernard H., Gross, Christian E., Bezard, Erwan
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Elsevier B.V 01.10.2004 Amsterdam Elsevier New York, NY
Témata:	Animals Biological and medical sciences Cell Count - methods Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases Disease Models, Animal Immunohistochemistry - methods Male Medical sciences Mesencephalon - injuries Mesencephalon - pathology Mice Mice, Inbred C57BL Neurology Neurons - metabolism Neuroprotection Parkinson Disease - metabolism Parkinson Disease - pathology Parkinson's disease Probability Rats Rats, Wistar Rodentia Substantia nigra pars compacta Tyrosine 3-Monooxygenase - metabolism Tyrosine hydroxylase J: Disorders of the nervous system Neuroprotection Tyrosine hydroxylase Parkinson's disease Substantia nigra pars compacta Animal model Rat Central nervous system Parkinson disease Tyrosine 3-monooxygenase Encephalon Degenerative disease Nervous system diseases Enzyme Rodentia Midbrain Cerebral disorder Vertebrata Mammalia Locus niger Asymmetry Mouse Central nervous system disease Oxidoreductases Lesion Extrapyramidal syndrome
ISSN:	0006-8993, 1872-6240
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Shrnutí:	Stereological counting of tyrosine-hydroxylase immunoreactive (TH-IR) neurons in the mesencephalon is a pivotal parameter in assessing the extent of lesioning in animal models of Parkinson's disease. We here show that the number of TH-IR neurons often appears abnormally decreased in healthy—commercially available—mice and rats, although both the number of Nissl-stained cells and the striatal dopaminergic innervation are unaffected. This potential bias in assessing extent of neurotoxin-induced lesion and subsequent protection by pharmacological manipulation prompts us to call for caution in setting up experimental designs.
Bibliografie:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0006-8993 1872-6240
DOI:	10.1016/j.brainres.2004.07.014