Investigating Vα7.2+/CD161− T Cell and MAIT Cell Profiles Using Flow Cytometry in Healthy Subjects and Subjects with Atopic Dermatitis

This study investigates the roles of mucosal-associated invariant T (MAIT) cells and Vα7.2+/CD161− T cells in skin diseases, focusing on atopic dermatitis. MAIT cells, crucial for bridging innate and adaptive immunity, were analyzed alongside Vα7.2+/CD161− T cells in peripheral blood samples from 14...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences Vol. 25; no. 6; p. 3486
Main Authors: Singh, Parvind, Gaspar, Krisztian, Szegedi, Andrea, Sajtos, Laszlo, Barath, Sandor, Hevessy, Zsuzsanna
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 20.03.2024
MDPI
Subjects:
Antigens
Atopic dermatitis
B cells
Dendritic cells
Dermatitis
Dermatitis, Atopic
Development and progression
Disease
Ethylenediaminetetraacetic acid
Flow Cytometry
Healthy Volunteers
Humans
Inflammation
Investigations
Lymphocytes
Medical equipment and supplies industry
Medical test kit industry
Mucosal-Associated Invariant T Cells
Pathogenesis
Skin
T cell receptors
T cells
Tumor Necrosis Factor-alpha
Tumor necrosis factor-TNF
United States
atopic dermatitis
dimensionality reduction
flow cytometry
unsupervised clustering
Vα7.2+/CD161− T cells
MAIT cells
ISSN:1422-0067, 1661-6596, 1422-0067
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study investigates the roles of mucosal-associated invariant T (MAIT) cells and Vα7.2+/CD161− T cells in skin diseases, focusing on atopic dermatitis. MAIT cells, crucial for bridging innate and adaptive immunity, were analyzed alongside Vα7.2+/CD161− T cells in peripheral blood samples from 14 atopic dermatitis patients and 10 healthy controls. Flow cytometry and machine learning algorithms were employed for a comprehensive analysis. The results indicate a significant decrease in MAIT cells and CD69 subsets in atopic dermatitis, coupled with elevated CD38 and polyfunctional MAIT cells producing TNFα and Granzyme B (TNFα+/GzB+). Vα7.2+/CD161− T cells in atopic dermatitis exhibited a decrease in CD8 and IFNγ-producing subsets but an increase in CD38 activated and IL-22-producing subsets. These results highlight the distinctive features of MAIT cells and Vα7.2+/CD161− T cells and their different roles in the pathogenesis of atopic dermatitis and provide insights into their potential roles in immune-mediated skin diseases.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
These authors contributed equally to this work.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25063486