Investigating Vα7.2+/CD161− T Cell and MAIT Cell Profiles Using Flow Cytometry in Healthy Subjects and Subjects with Atopic Dermatitis

This study investigates the roles of mucosal-associated invariant T (MAIT) cells and Vα7.2+/CD161− T cells in skin diseases, focusing on atopic dermatitis. MAIT cells, crucial for bridging innate and adaptive immunity, were analyzed alongside Vα7.2+/CD161− T cells in peripheral blood samples from 14...

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Vydáno v:International journal of molecular sciences Ročník 25; číslo 6; s. 3486
Hlavní autoři: Singh, Parvind, Gaspar, Krisztian, Szegedi, Andrea, Sajtos, Laszlo, Barath, Sandor, Hevessy, Zsuzsanna
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland MDPI AG 20.03.2024
MDPI
Témata:
Antigens
Atopic dermatitis
B cells
Dendritic cells
Dermatitis
Dermatitis, Atopic
Development and progression
Disease
Ethylenediaminetetraacetic acid
Flow Cytometry
Healthy Volunteers
Humans
Inflammation
Investigations
Lymphocytes
Medical equipment and supplies industry
Medical test kit industry
Mucosal-Associated Invariant T Cells
Pathogenesis
Skin
T cell receptors
T cells
Tumor Necrosis Factor-alpha
Tumor necrosis factor-TNF
United States
atopic dermatitis
dimensionality reduction
flow cytometry
unsupervised clustering
Vα7.2+/CD161− T cells
MAIT cells
ISSN:1422-0067, 1661-6596, 1422-0067
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Shrnutí:This study investigates the roles of mucosal-associated invariant T (MAIT) cells and Vα7.2+/CD161− T cells in skin diseases, focusing on atopic dermatitis. MAIT cells, crucial for bridging innate and adaptive immunity, were analyzed alongside Vα7.2+/CD161− T cells in peripheral blood samples from 14 atopic dermatitis patients and 10 healthy controls. Flow cytometry and machine learning algorithms were employed for a comprehensive analysis. The results indicate a significant decrease in MAIT cells and CD69 subsets in atopic dermatitis, coupled with elevated CD38 and polyfunctional MAIT cells producing TNFα and Granzyme B (TNFα+/GzB+). Vα7.2+/CD161− T cells in atopic dermatitis exhibited a decrease in CD8 and IFNγ-producing subsets but an increase in CD38 activated and IL-22-producing subsets. These results highlight the distinctive features of MAIT cells and Vα7.2+/CD161− T cells and their different roles in the pathogenesis of atopic dermatitis and provide insights into their potential roles in immune-mediated skin diseases.
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These authors contributed equally to this work.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25063486