The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites

Systemic infections are commonly associated with changes in host metabolism and gene expression. Sunflower chlorotic mottle virus (SuCMoV) causes systemic infection with sugar increase, photoinhibition and increase in antioxidant enzyme activities before chlorotic symptom appearance in sunflower lea...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:Plant science (Limerick) Ročník 196; s. 107 - 116
Hlavní autori: Rodríguez, Marianela, Muñoz, Nacira, Lenardon, Sergio, Lascano, Ramiro
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Shannon Elsevier Ireland Ltd 01.11.2012
Elsevier
Predmet:
adenosine triphosphate
Adenosine Triphosphate - metabolism
Biological and medical sciences
catalase
Chlorophyll
Chlorophyll - metabolism
enzyme activity
Fundamental and applied biological sciences. Psychology
Gene expression
gene expression regulation
Gene Expression Regulation, Plant
genes
genetics
glutathione
Glutathione - metabolism
Helianthus
Helianthus - genetics
Helianthus - metabolism
Helianthus - virology
Helianthus annuus
homeostasis
Host-Pathogen Interactions
hydrogen peroxide
Hydrogen Peroxide - metabolism
iron
leaves
manganese
metabolism
metabolites
Mitochondrial Proteins
Mitochondrial Proteins - genetics
NADP
NADP - metabolism
Oxidation-Reduction
Oxidoreductases
Oxidoreductases - genetics
photoinhibition
Photosystem I Protein Complex
Photosystem I Protein Complex - genetics
Photosystem II Protein Complex
Photosystem II Protein Complex - genetics
physiology
Phytopathology. Animal pests. Plant and forest protection
Plant Diseases
Plant Diseases - genetics
Plant Diseases - virology
Plant Leaves
Plant Leaves - genetics
Plant Leaves - metabolism
Plant Leaves - virology
Plant Proteins
Plant Proteins - genetics
Plant viruses and viroids
Potyvirus
Potyvirus - physiology
pyridine nucleotides
Redox state
Reverse Transcriptase Polymerase Chain Reaction
SuCMoV
sugars
Sunflower
Superoxide Dismutase
Superoxide Dismutase - genetics
Symptom development
Time Factors
virology
viruses
zinc
SuCMoV
psbO
DTT
LS
APX
BS
FW
psbA
cp29
GSH
Symptom development
DGC
GSSG
Redox state
PVPP
SDS
DTNB
GO
SOD
AOX
DEPC
fnr
GR
EDTA
OEC
Gene expression
ES
PEG
TCA
rbcS
CAT
ROS
Sunflower
NAD(P)
psaH
Oxidation reduction
Plant pathogen
Metabolite
Plant biology
Compositae
Virus
Chlorosis
Symptomatology
Helianthus annuus
Potato virus Y
Dicotyledones
Angiospermae
Development
Spermatophyta
Potyvirus
Oil plant (vegetal)
Potyviridae
ISSN:0168-9452, 1873-2259, 1873-2259
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Systemic infections are commonly associated with changes in host metabolism and gene expression. Sunflower chlorotic mottle virus (SuCMoV) causes systemic infection with sugar increase, photoinhibition and increase in antioxidant enzyme activities before chlorotic symptom appearance in sunflower leaves. The aim of this study was to determine if chlorotic symptom development induced by SuCMoV infection is accompanied by changes in different redox-related metabolites and transcripts. Symptom development was analyzed in the second pair of leaves (systemic infection) at different post-inoculation times: before symptom appearance (BS, 4dpi), and at an early (ES, 7dpi) and later stage (LS, 12dpi) of symptom expression. The results showed that the virus reaches the second pair of leaves at 4dpi. A positive correlation between chlorotic symptom and number of viral copies was also observed. Changes in hydrogen peroxide, glutathione, pyridine nucleotides and ATP content were observed since symptom appearance (ES, 7dpi). The expression of some of the genes analyzed was also strongly affected by SuCMoV infection. Specifically, down-regulation of both chloroplast-encoded genes and chloroplast-targeted genes: psbA, rbcS, Cu/Zn sod, Fe sod, phosphoglycolate phosphatase, psbO, psaH and fnr was present, whereas the expression of cytoplasmic-targeted genes, apx1, and Cu/Zn sod was up-regulated. Mitochondrial Mn sod decreased at BS stage and aox decreased only at ES stage. Peroxisomal catalase (cat-2) was lower at BS and LS stages. All these results suggest that SuCMoV infection induces progressive changes in determinants of redox homeostasis associated with chlorotic symptom development.
Bibliografia:http://dx.doi.org/10.1016/j.plantsci.2012.08.008
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0168-9452
1873-2259
1873-2259
DOI:10.1016/j.plantsci.2012.08.008