High resolution single particle Cryo-EM refinement using JSPR

JSPR is a single particle cryo-EM image processing and 3D reconstruction software developed in the Jiang laboratory at Purdue University. It began as a few refinement scripts for symmetric and asymmetric reconstructions of icosahedral viruses, but has grown into a comprehensive suite of tools for bu...

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Vydáno v:Progress in biophysics and molecular biology Ročník 160; s. 37 - 42
Hlavní autoři: Sun, Chen, Gonzalez, Brenda, Vago, Frank S., Jiang, Wen
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Elsevier Ltd 01.03.2021
Témata:
Aberration correction
Bacteriophages - chemistry
Beam tilt
Cryoelectron Microscopy - methods
Defocus refinement
Elliptic distortion
Enterovirus - chemistry
Generalized 2D alignments
Image Processing, Computer-Assisted
Imaging, Three-Dimensional
Phase shift refinement
Protein Conformation
Single Molecule Imaging - methods
Software
Viruses - chemistry
Zika Virus - chemistry
Aberration correction
Generalized 2D alignments
Defocus refinement
Elliptic distortion
Beam tilt
Phase shift refinement
ISSN:0079-6107, 1873-1732, 1873-1732
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Popis
Shrnutí:JSPR is a single particle cryo-EM image processing and 3D reconstruction software developed in the Jiang laboratory at Purdue University. It began as a few refinement scripts for symmetric and asymmetric reconstructions of icosahedral viruses, but has grown into a comprehensive suite of tools for building ab initio reconstructions, high resolution refinements of viruses, protein complexes of arbitrary symmetries including helical tubes/filaments, and image file handling utilities. In this review, we will present examples achieved using JSPR and demonstrate recently implemented features of JSPR such as multi-aberration "alignments" and automatic optimization of masking for the assessment of map resolution using "true" FSC.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ObjectType-Review-3
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Chen Sun: Writing - Original Draft, Visualization Brenda Gonzalez: Writing - Review & Editing Frank S. Vago: Writing - Review & Editing Wen Jiang: Conceptualization, Methodology, Software, Supervision, Funding acquisition, Writing - Review & Editing
ISSN:0079-6107
1873-1732
1873-1732
DOI:10.1016/j.pbiomolbio.2020.05.006