Characterization of bluetongue virus ribonucleic acid

An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and int...

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Bibliographic Details
Published in:Journal of virology Vol. 5; no. 1; p. 1
Main Authors: Verwoerd, D W, Louw, H, Oellermann, R A
Format: Journal Article
Language:English
Published: United States 01.01.1970
Subjects:
Acrylates
Animals
Bluetongue - etiology
Carbon Isotopes
Cell Line
Centrifugation, Density Gradient
Chromatography
Cricetinae
Electrophoresis
Formaldehyde
Gels
Hot Temperature
Indicators and Reagents
Kidney
L Cells (Cell Line)
Molecular Weight
Nucleic Acid Denaturation
Phenols
Phosphorus Isotopes
Reoviridae - analysis
RNA, Viral - analysis
RNA, Viral - isolation & purification
Sheep
Spectrophotometry
Sucrose
Tritium
Uridine
Viruses, Unclassified - isolation & purification
ISSN:0022-538X
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Summary:An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.
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ISSN:0022-538X
DOI:10.1128/jvi.5.1.1-7.1970