Fructose-fed streptozotocin-injected rat: an alternative model for type 2 diabetes

The main objective of the study was to develop an alternative non-genetic rat model for type 2 diabetes (T2D). Six-week-old male Sprague-Dawley rats (190.56±23.60 g) were randomly divided into six groups, namely: Normal Control (NC), Diabetic Control (DBC), Fructose-10 (FR10), Fructose-20 (FR20), Fr...

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Published in:Pharmacological reports Vol. 64; no. 1; pp. 129 - 139
Main Authors: Wilson, Rachel D., Islam, Md. Shahidul
Format: Journal Article
Language:English
Published: Cham Elsevier Urban & Partner Sp. z o.o 01.01.2012
Springer International Publishing
Subjects:
animal model
Animals
Blood Glucose - metabolism
Diabetes Mellitus, Experimental - blood
Diabetes Mellitus, Experimental - chemically induced
Diabetes Mellitus, Experimental - metabolism
Diabetes Mellitus, Type 2 - blood
Diabetes Mellitus, Type 2 - metabolism
Diet
Disease Models, Animal
Drinking Water
Drug Safety and Pharmacovigilance
Eating
experimental diabetes
fructose
Fructose - administration & dosage
Glucose Tolerance Test - methods
Insulin - blood
Insulin - metabolism
Insulin Resistance - physiology
Insulin-Secreting Cells - metabolism
Lipids - blood
Liver Glycogen - metabolism
Male
Pharmacotherapy
Pharmacy
rat
Rats
Rats, Sprague-Dawley
streptozotocin
type 2 diabetes
fructose
type 2 diabetes
streptozotocin
rat
experimental diabetes
animal model
ISSN:1734-1140, 2299-5684, 2299-5684
Online Access:Get full text
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Summary:The main objective of the study was to develop an alternative non-genetic rat model for type 2 diabetes (T2D). Six-week-old male Sprague-Dawley rats (190.56±23.60 g) were randomly divided into six groups, namely: Normal Control (NC), Diabetic Control (DBC), Fructose-10 (FR10), Fructose-20 (FR20), Fructose-30 (FR30) and Fructose-40 (FR40) and were fed a normal rat pellet diet ad libitum for 2 weeks. During this period, the two control groups received normal drinking water whilst the fructose groups received 10, 20, 30 and 40% fructose in drinking water ad libitum, respectively. After two weeks of dietary manipulation, all groups except the NC group received a single injection (ip) of streptozotocin (STZ) (40mg/kg b.w.) dissolved in citrate buffer (pH 4.4). The NC group received only a vehicle buffer injection (ip). One week after the STZ injection, animals with non-fasting blood glucose levels > 300mg/dl were considered as diabetic. Three weeks after the STZ injection, the animals in FR20, FR30 and FR40 groups were eliminated from the study due to the severity of diabetes and the FR10 group was selected for the remainder of the 11 weeks experimental period. The significantly (p<0.05) higher fluid intake, blood glucose, serum lipids, liver glycogen, liver function enzymes and insulin resistance (HOMA-IR) and significantly (p<0.05) lower body weight, oral glucose tolerance, number of pancreatic β-cells and pancreatic β-cell functions (HOMA-β) of FR10 group demonstrate that the 10% fructose-fed followed by 40mg/kg of BW STZ injected rat can be a new and alternative model for T2D.
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ISSN:1734-1140
2299-5684
2299-5684
DOI:10.1016/S1734-1140(12)70739-9