New Insights to Regulation of Fructose-1,6-bisphosphatase during Anoxia in Red-Eared Slider, Trachemys scripta elegans

The red-eared slider (Trachemys scripta elegans) undergoes numerous changes to its physiological and metabolic processes to survive without oxygen. During anoxic conditions, its metabolic rate drops drastically to minimize energy requirements. The alterations in the central metabolic pathways are of...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Vol. 11; no. 10; p. 1548
Main Authors: Gupta, Aakriti, Varma, Anchal, Storey, Kenneth B.
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 19.10.2021
MDPI
Subjects:
Anaerobic conditions
Animals
Anoxia
Dehydrogenases
Energy requirements
enzyme regulation
Enzymes
Fructose
Fructose - genetics
Fructose - metabolism
Fructose-1,6-bisphosphatse
Fructose-1,6-diphosphate
Fructose-Bisphosphatase - metabolism
Gluconeogenesis
Glucose
Glycerol
Homogenization
Hypoxia - genetics
Hypoxia - metabolism
Hypoxia - pathology
Immunoblotting
Kinases
Liver
Liver - metabolism
Metabolic pathways
Metabolic rate
metabolic rate depression
Metabolism
Oxygen - metabolism
Phosphorylation
Phosphorylation - genetics
Post-translation
Protein Processing, Post-Translational
Proteins
Signal Transduction - genetics
Threonine
Trachemys scripta elegans
Turtles - metabolism
Canada
United States > US
Quebec Canada
Fructose-1,6-bisphosphatse
metabolic rate depression
Trachemys scripta elegans
anoxia
enzyme regulation
post-translational modification
gluconeogenesis
ISSN:2218-273X, 2218-273X
Online Access:Get full text
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Summary:The red-eared slider (Trachemys scripta elegans) undergoes numerous changes to its physiological and metabolic processes to survive without oxygen. During anoxic conditions, its metabolic rate drops drastically to minimize energy requirements. The alterations in the central metabolic pathways are often accomplished by the regulation of key enzymes. The regulation of one such enzyme, fructose-1,6-bisphosphatase (FBPase; EC 3.1.3.11), was characterized in the present study during anoxia in liver. FBPase is a crucial enzyme of gluconeogenesis. The FBPase was purified from liver tissue in both control and anoxic conditions and subsequently assayed to determine the kinetic parameters of the enzyme. The study revealed the relative degree of post-translational modifications in the FBPase from control and anoxic turtles. Further, this study demonstrated a significant decrease in the maximal activity in anoxic FBPase and decreased sensitivity to its substrate Fructose-1,6-bisphosphate (FBP) when compared to the control. Immunoblotting demonstrated increased threonine phosphorylation (~1.4-fold) in the anoxic FBPase. Taken together, these results suggest that the phosphorylation of liver FBPase is an important step in suppressing FBPase activity, ultimately leading to the inhibition of gluconeogenesis in the liver of the red-eared slider during anaerobic conditions.
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ISSN:2218-273X
2218-273X
DOI:10.3390/biom11101548