The Role of Surface Nanotopography and Chemistry on Primary Neutrophil and Macrophage Cellular Responses

Synthetic materials employed for enhancing, replacing, or restoring biological functionality may be compromised by the host immune responses that they evoke. Surface modification has attracted substantial attention as a tool to modulate the host response to synthetic materials; however, how surface...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Advanced healthcare materials Ročník 5; číslo 8; s. 956 - 965
Hlavní autoři: Christo, Susan N., Bachhuka, Akash, Diener, Kerrilyn R., Mierczynska, Agnieszka, Hayball, John D., Vasilev, Krasimir
Médium: Journal Article
Jazyk:angličtina
Vydáno: Germany Blackwell Publishing Ltd 20.04.2016
Wiley Subscription Services, Inc
Témata:
Acids - pharmacology
Amines
Animals
Cell Line
Cells, Cultured
Control surfaces
Cytokines - secretion
Effectors
immunomodulation
Inflammation Mediators - metabolism
Lead (metal)
Macrophages
Macrophages - drug effects
Matrix Metalloproteinase 9 - biosynthesis
Mice, Inbred C57BL
Microscopy, Atomic Force
Nanoparticles
Nanoparticles - chemistry
Nanostructure
Nanotechnology - methods
Neutrophil Activation
Neutrophils
Neutrophils - cytology
Photoelectron Spectroscopy
surface chemistries
Surface chemistry
surface nanotopographies
Surface Properties
Synthetic products
T cell receptors
immunomodulation
macrophages
surface nanotopographies
neutrophils
surface chemistries
ISSN:2192-2640, 2192-2659, 2192-2659
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:Synthetic materials employed for enhancing, replacing, or restoring biological functionality may be compromised by the host immune responses that they evoke. Surface modification has attracted substantial attention as a tool to modulate the host response to synthetic materials; however, how surface nanotopography combined with chemistry affects immune effector cell responses is still poorly understood. To address this open question, a unique set of model surfaces with controlled surface nanotopography in the range of 16, 38, and 68 nm has been generated. Tailored outermost surface chemistry that was amine, carboxyl, or methyl group rich has been provided. The combinations of these properties yield 12 surface types that are subject to functional assays assessing key immune effector cells, namely, primary neutrophil and macrophage responses in vitro. The data demonstrate that surface nanotopography leads to enhanced matrix metalloproteinase‐9 production from primary neutrophils, and a decrease in pro‐inflammatory cytokine secretion from primary macrophages. Together, these results are the first to directly compare the immunomodulatory effects of the cooperative interplay between surface nanotopography and chemistry. Innate immune effector cells can differentially respond to the controlled surface nanotopography in the range of 16, 38, and 68 nm. Additional overcoating of these surfaces with amine, carboxyl, or methyl group rich chemistries demonstrates that surfaces with hydrophillic anionic overcoated 68 nm gold nanoparticles can modulate neutrophil and macrophage functionality.
Bibliografie:istex:515C6E1697CD011C792B473F8771763E5E5F07C8
ARC - No. DP15104212
ark:/67375/WNG-W5TRG9JV-S
ArticleID:ADHM201500845
NHMRC - No. PG631931
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2192-2640
2192-2659
2192-2659
DOI:10.1002/adhm.201500845