Novel secretory protein Ss-Caf1 of the plant-pathogenic fungus Sclerotinia sclerotiorum is required for host penetration and normal sclerotial development

To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional library. Sunf-MT6 could not form compound appressorium and failed to induce lesions on leaves of rapeseed though it could produce more oxalic acid...

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Vydáno v:	Molecular plant-microbe interactions Ročník 27; číslo 1; s. 40 - 55
Hlavní autoři:	Xiao, Xueqiong, Xie, Jiatao, Cheng, Jiasen, Li, Guoqing, Yi, Xianhong, Jiang, Daohong, Fu, Yanping
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States The American Phytopathological Society 01.01.2014
Témata:	Amino Acid Motifs Arabidopsis - microbiology Arabidopsis - physiology Ascomycota - genetics Ascomycota - pathogenicity Ascomycota - physiology Ascomycota - ultrastructure Cell Death Daucus carota - microbiology Daucus carota - physiology Fruiting Bodies, Fungal Fungal Proteins - genetics Fungal Proteins - metabolism Gene Library Gene Silencing Host-Pathogen Interactions Models, Molecular Mutagenesis, Insertional Nicotiana - microbiology Nicotiana - physiology Oxalic Acid - metabolism Phenotype Phylogeny Plant Diseases - microbiology Plant Leaves - microbiology Plant Leaves - physiology Plant Roots - microbiology Plant Roots - physiology Seedlings - microbiology Seedlings - physiology Stress, Physiological
ISSN:	0894-0282, 1943-7706
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Shrnutí:	To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional library. Sunf-MT6 could not form compound appressorium and failed to induce lesions on leaves of rapeseed though it could produce more oxalic acid than the wild-type strain. However, it could enter into host tissues via wounds and cause typical necrotic lesions. Furthermore, Sunf-MT6 produced fewer but larger sclerotia than the wild-type strain Sunf-M. A gene, named Ss-caf1, was disrupted by T-DNA insertion in Sunf-MT6. Gene complementation and knockdown experiments confirmed that the disruption of Ss-caf1 was responsible for the phenotypic changes of Sunf-MT6. Ss-caf1 encodes a secretory protein with a putative Ca(2+)-binding EF-hand motif. High expression levels of Ss-caf1 were observed at an early stage of compound appressorium formation and in immature sclerotia. Expression of Ss-caf1 without signal peptides in Nicotiana benthamiana via Tobacco rattle virus-based vectors elicited cell death. These results suggest that Ss-caf1 plays an important role in compound appressorium formation and sclerotial development of S. sclerotiorum. In addition, Ss-Caf1 has the potential to interact with certain host proteins or unknown substances in host cells, resulting in subsequent host cell death.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0894-0282 1943-7706
DOI:	10.1094/MPMI-05-13-0145-R