Identification of three novel mycoplasma species from ostriches in South Africa

Mycoplasmas have been implicated in certain clinical syndromes in ostriches and are associated with upper respiratory tract infections. As these infections result in production losses, they are of considerable economic importance to the South African ostrich industry. Although poultry mycoplasmas ha...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	Veterinary microbiology Ročník 111; číslo 3; s. 159 - 169
Hlavní autori:	Botes, A., Peyrot, B.M., Olivier, A.J., Burger, W.P., Bellstedt, D.U.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Amsterdam Elsevier B.V 20.12.2005 Elsevier Science
Predmet:	Animals Bacteriology Base Sequence Biological and medical sciences bird diseases Bird Diseases - epidemiology Bird Diseases - microbiology Bird Diseases - transmission chickens Chickens - microbiology disease detection disease diagnosis disease transmission DNA, Bacterial - analysis DNA, Bacterial - isolation & purification Female Fundamental and applied biological sciences. Psychology Gene Amplification Male Microbiology Miscellaneous Molecular Sequence Data Mycoplasma Mycoplasma - classification Mycoplasma - isolation & purification Mycoplasma Infections - epidemiology Mycoplasma Infections - microbiology Mycoplasma Infections - transmission Mycoplasma Infections - veterinary mycoplasmosis nucleotide sequences Ostrich Ostrich disease ostriches pathogen identification Phylogeny respiratory tract diseases RNA, Ribosomal, 16S - chemistry RNA, Ribosomal, 16S - genetics Sequence Alignment - veterinary South Africa South Africa - epidemiology Species Specificity Specific PCR Struthioniformes - microbiology South Africa Africa Ostrich disease Mycoplasma Ostrich Specific PCR Mollicutes Polymerase chain reaction Mycoplasmatales Microbiology Bacteria Identification Veterinary Mycoplasmataceae
ISSN:	0378-1135, 1873-2542
On-line prístup:	Získať plný text
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Popis
Shrnutí:	Mycoplasmas have been implicated in certain clinical syndromes in ostriches and are associated with upper respiratory tract infections. As these infections result in production losses, they are of considerable economic importance to the South African ostrich industry. Although poultry mycoplasmas have been shown to infect ostriches, the existence of unique ostrich-specific mycoplasmas has been suggested. In this study, mycoplasmas were isolated from ostriches in the Klein Karoo, Central Karoo and Garden Route areas of the Western and Northern Cape Provinces of South Africa and identified using 16S rRNA gene sequencing. These sequences indicated that ostriches in these areas carry three unique mycoplasmas and were not infected with chicken mycoplasmas. Phylogenetic analysis of the 16S rRNA sequences of the three isolated ostrich mycoplasmas showed them to be quite divergent and to fall into two distinct phylogenetic groupings. Unique sequences within the 16S rRNA gene of the ostrich mycoplasmas were subsequently used for the development of specific primers for the detection and diagnosis of mycoplasma infections in ostriches. Chickens kept in close proximity to infected ostriches were not infected with these ostrich mycoplasmas.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-1135 1873-2542
DOI:	10.1016/j.vetmic.2005.10.017