Identification of three novel mycoplasma species from ostriches in South Africa

Mycoplasmas have been implicated in certain clinical syndromes in ostriches and are associated with upper respiratory tract infections. As these infections result in production losses, they are of considerable economic importance to the South African ostrich industry. Although poultry mycoplasmas ha...

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Bibliographic Details
Published in:Veterinary microbiology Vol. 111; no. 3; pp. 159 - 169
Main Authors: Botes, A., Peyrot, B.M., Olivier, A.J., Burger, W.P., Bellstedt, D.U.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 20.12.2005
Elsevier Science
Subjects:
Animals
Bacteriology
Base Sequence
Biological and medical sciences
bird diseases
Bird Diseases - epidemiology
Bird Diseases - microbiology
Bird Diseases - transmission
chickens
Chickens - microbiology
disease detection
disease diagnosis
disease transmission
DNA, Bacterial - analysis
DNA, Bacterial - isolation & purification
Female
Fundamental and applied biological sciences. Psychology
Gene Amplification
Male
Microbiology
Miscellaneous
Molecular Sequence Data
Mycoplasma
Mycoplasma - classification
Mycoplasma - isolation & purification
Mycoplasma Infections - epidemiology
Mycoplasma Infections - microbiology
Mycoplasma Infections - transmission
Mycoplasma Infections - veterinary
mycoplasmosis
nucleotide sequences
Ostrich
Ostrich disease
ostriches
pathogen identification
Phylogeny
respiratory tract diseases
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
Sequence Alignment - veterinary
South Africa
South Africa - epidemiology
Species Specificity
Specific PCR
Struthioniformes - microbiology
South Africa
Africa
Ostrich disease
Mycoplasma
Ostrich
Specific PCR
Mollicutes
Polymerase chain reaction
Mycoplasmatales
Microbiology
Bacteria
Identification
Veterinary
Mycoplasmataceae
ISSN:0378-1135, 1873-2542
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Summary:Mycoplasmas have been implicated in certain clinical syndromes in ostriches and are associated with upper respiratory tract infections. As these infections result in production losses, they are of considerable economic importance to the South African ostrich industry. Although poultry mycoplasmas have been shown to infect ostriches, the existence of unique ostrich-specific mycoplasmas has been suggested. In this study, mycoplasmas were isolated from ostriches in the Klein Karoo, Central Karoo and Garden Route areas of the Western and Northern Cape Provinces of South Africa and identified using 16S rRNA gene sequencing. These sequences indicated that ostriches in these areas carry three unique mycoplasmas and were not infected with chicken mycoplasmas. Phylogenetic analysis of the 16S rRNA sequences of the three isolated ostrich mycoplasmas showed them to be quite divergent and to fall into two distinct phylogenetic groupings. Unique sequences within the 16S rRNA gene of the ostrich mycoplasmas were subsequently used for the development of specific primers for the detection and diagnosis of mycoplasma infections in ostriches. Chickens kept in close proximity to infected ostriches were not infected with these ostrich mycoplasmas.
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ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2005.10.017