Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation

Brown adipose tissue (BAT) undergoes pronounced changes after birth coincident with the loss of the BAT-specific uncoupling protein (UCP)1 and rapid fat growth. The extent to which this adaptation may vary between anatomical locations remains unknown, or whether the process is sensitive to maternal...

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Vydané v:Scientific reports Ročník 8; číslo 1; s. 9628 - 11
Hlavní autori: Fainberg, Hernan P., Birtwistle, Mark, Alagal, Reham, Alhaddad, Ahmad, Pope, Mark, Davies, Graeme, Woods, Rachel, Castellanos, Marcos, May, Sean T., Ortori, Catharine A., Barrett, David A., Perry, Viv, Wiens, Frank, Stahl, Bernd, van der Beek, Eline, Sacks, Harold, Budge, Helen, Symonds, Michael E.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 25.06.2018
Nature Publishing Group
Predmet:
631/114/2164
631/443/319/2723
Adipose tissue
Adipose tissue (brown)
Adipose Tissue, Brown - drug effects
Adipose Tissue, Brown - growth & development
Adipose Tissue, Brown - metabolism
Adipose Tissue, White - drug effects
Adipose Tissue, White - growth & development
Adipose Tissue, White - metabolism
Age
Animals
Canola
Canola oil
Cytochrome
Cytochrome c
Data Mining
Data processing
Diet
Dietary Supplements
Feed additives
Female
Gene expression
Gene Regulatory Networks - drug effects
Genomes
Humanities and Social Sciences
Laboratories
Learning algorithms
Machine learning
Metabolism
Milk
Mitochondria
Mitochondrial Proteins - genetics
Mitochondrial Proteins - metabolism
Mothers
multidisciplinary
Multigene Family - genetics
Nutrition research
Proteins
Science
Science (multidisciplinary)
Sheep
Transcription
Transcription, Genetic - drug effects
ISSN:2045-2322, 2045-2322
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Shrnutí:Brown adipose tissue (BAT) undergoes pronounced changes after birth coincident with the loss of the BAT-specific uncoupling protein (UCP)1 and rapid fat growth. The extent to which this adaptation may vary between anatomical locations remains unknown, or whether the process is sensitive to maternal dietary supplementation. We, therefore, conducted a data mining based study on the major fat depots (i.e. epicardial, perirenal, sternal (which possess UCP1 at 7 days), subcutaneous and omental) (that do not possess UCP1) of young sheep during the first month of life. Initially we determined what effect adding 3% canola oil to the maternal diet has on mitochondrial protein abundance in those depots which possessed UCP1. This demonstrated that maternal dietary supplementation delayed the loss of mitochondrial proteins, with the amount of cytochrome C actually being increased. Using machine learning algorithms followed by weighted gene co-expression network analysis, we demonstrated that each depot could be segregated into a unique and concise set of modules containing co-expressed genes involved in adipose function. Finally using lipidomic analysis following the maternal dietary intervention, we confirmed the perirenal depot to be most responsive. These insights point at new research avenues for examining interventions to modulate fat development in early life.
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SourceType-Scholarly Journals-1
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-27376-3