Integrative structure of a 10-megadalton eukaryotic pyruvate dehydrogenase complex from native cell extracts

The pyruvate dehydrogenase complex (PDHc) is a giant enzymatic assembly involved in pyruvate oxidation. PDHc components have been characterized in isolation, but the complex’s quaternary structure has remained elusive due to sheer size, heterogeneity, and plasticity. Here, we identify fully assemble...

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Bibliographic Details
Published in:	Cell reports (Cambridge) Vol. 34; no. 6; p. 108727
Main Authors:	Kyrilis, Fotis L., Semchonok, Dmitry A., Skalidis, Ioannis, Tüting, Christian, Hamdi, Farzad, O’Reilly, Francis J., Rappsilber, Juri, Kastritis, Panagiotis L.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 09.02.2021 Elsevier
Subjects:	Cell Extracts - chemistry cellular homogenates Chaetomium - enzymology cryo-EM Cryoelectron Microscopy Fungal Proteins - chemistry Fungal Proteins - isolation & purification image processing integrative structural biology keto acid dehydrogenase complexes Models, Molecular native cell extracts negative staining Protein Structure, Quaternary Pyruvate Dehydrogenase Complex - chemistry Pyruvate Dehydrogenase Complex - isolation & purification pyruvate dehydrogenase factory model image processing cellular homogenates native cell extracts pyruvate dehydrogenase factory model integrative structural biology negative staining keto acid dehydrogenase complexes cryo-EM
ISSN:	2211-1247, 2211-1247
Online Access:	Get full text
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