Development and validation of a targeted gene sequencing panel for application to disparate cancers

Next generation sequencing has revolutionised genomic studies of cancer, having facilitated the development of precision oncology treatments based on a tumour’s molecular profile. We aimed to develop a targeted gene sequencing panel for application to disparate cancer types with particular focus on...

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Published in:	Scientific reports Vol. 9; no. 1; pp. 17052 - 16
Main Authors:	McCabe, Mark J., Gauthier, Marie-Emilie A., Chan, Chia-Ling, Thompson, Tanya J., De Sousa, Sunita M.C., Puttick, Clare, Grady, John P., Gayevskiy, Velimir, Tao, Jiang, Ying, Kevin, Cipponi, Arcadi, Deng, Niantao, Swarbrick, Alex, Thomas, Melissa L., Lord, Reginald V., Johns, Amber L., Kohonen-Corish, Maija, O’Toole, Sandra A., Clark, Jonathan, Mueller, Simon A., Gupta, Ruta, McCormack, Ann I., Dinger, Marcel E., Cowley, Mark J.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 19.11.2019 Nature Publishing Group
Subjects:	45/23 45/41 45/47 631/208/514/2254 631/67/69 692/4028/67/69 Bioinformatics Biomarkers, Tumor - genetics Biopsy Cancer Carcinoma, Pancreatic Ductal - genetics Cell Line, Tumor Circulating Tumor DNA - genetics Computational Biology - methods Deoxyribonucleic acid DNA DNA sequencing Gene frequency Genetic Predisposition to Disease - genetics Genetic testing Genomics - methods Head & neck cancer Head and Neck Neoplasms - genetics High-Throughput Nucleotide Sequencing - methods Humanities and Social Sciences Humans Liquid Biopsy multidisciplinary Next-generation sequencing Oncology Pancreatic Neoplasms - genetics Pituitary Neoplasms - genetics Precision medicine Precision Medicine - methods Science Science (multidisciplinary) Sensitivity and Specificity Squamous Cell Carcinoma of Head and Neck - genetics Tumors
ISSN:	2045-2322, 2045-2322
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Abstract	Next generation sequencing has revolutionised genomic studies of cancer, having facilitated the development of precision oncology treatments based on a tumour’s molecular profile. We aimed to develop a targeted gene sequencing panel for application to disparate cancer types with particular focus on tumours of the head and neck, plus test for utility in liquid biopsy. The final panel designed through Roche/Nimblegen combined 451 cancer-associated genes (2.01 Mb target region). 136 patient DNA samples were collected for performance and application testing. Panel sensitivity and precision were measured using well-characterised DNA controls (n = 47), and specificity by Sanger sequencing of the Aryl Hydrocarbon Receptor Interacting Protein ( AIP ) gene in 89 patients. Assessment of liquid biopsy application employed a pool of synthetic circulating tumour DNA (ctDNA). Library preparation and sequencing were conducted on Illumina-based platforms prior to analysis with our accredited (ISO15189) bioinformatics pipeline. We achieved a mean coverage of 395x, with sensitivity and specificity of >99% and precision of >97%. Liquid biopsy revealed detection to 1.25% variant allele frequency. Application to head and neck tumours/cancers resulted in detection of mutations aligned to published databases. In conclusion, we have developed an analytically-validated panel for application to cancers of disparate types with utility in liquid biopsy.
AbstractList	Next generation sequencing has revolutionised genomic studies of cancer, having facilitated the development of precision oncology treatments based on a tumour’s molecular profile. We aimed to develop a targeted gene sequencing panel for application to disparate cancer types with particular focus on tumours of the head and neck, plus test for utility in liquid biopsy. The final panel designed through Roche/Nimblegen combined 451 cancer-associated genes (2.01 Mb target region). 136 patient DNA samples were collected for performance and application testing. Panel sensitivity and precision were measured using well-characterised DNA controls (n = 47), and specificity by Sanger sequencing of the Aryl Hydrocarbon Receptor Interacting Protein ( AIP ) gene in 89 patients. Assessment of liquid biopsy application employed a pool of synthetic circulating tumour DNA (ctDNA). Library preparation and sequencing were conducted on Illumina-based platforms prior to analysis with our accredited (ISO15189) bioinformatics pipeline. We achieved a mean coverage of 395x, with sensitivity and specificity of >99% and precision of >97%. Liquid biopsy revealed detection to 1.25% variant allele frequency. Application to head and neck tumours/cancers resulted in detection of mutations aligned to published databases. In conclusion, we have developed an analytically-validated panel for application to cancers of disparate types with utility in liquid biopsy. Next generation sequencing has revolutionised genomic studies of cancer, having facilitated the development of precision oncology treatments based on a tumour's molecular profile. We aimed to develop a targeted gene sequencing panel for application to disparate cancer types with particular focus on tumours of the head and neck, plus test for utility in liquid biopsy. The final panel designed through Roche/Nimblegen combined 451 cancer-associated genes (2.01 Mb target region). 136 patient DNA samples were collected for performance and application testing. Panel sensitivity and precision were measured using well-characterised DNA controls (n = 47), and specificity by Sanger sequencing of the Aryl Hydrocarbon Receptor Interacting Protein (AIP) gene in 89 patients. Assessment of liquid biopsy application employed a pool of synthetic circulating tumour DNA (ctDNA). Library preparation and sequencing were conducted on Illumina-based platforms prior to analysis with our accredited (ISO15189) bioinformatics pipeline. We achieved a mean coverage of 395x, with sensitivity and specificity of >99% and precision of >97%. Liquid biopsy revealed detection to 1.25% variant allele frequency. Application to head and neck tumours/cancers resulted in detection of mutations aligned to published databases. In conclusion, we have developed an analytically-validated panel for application to cancers of disparate types with utility in liquid biopsy.Next generation sequencing has revolutionised genomic studies of cancer, having facilitated the development of precision oncology treatments based on a tumour's molecular profile. We aimed to develop a targeted gene sequencing panel for application to disparate cancer types with particular focus on tumours of the head and neck, plus test for utility in liquid biopsy. The final panel designed through Roche/Nimblegen combined 451 cancer-associated genes (2.01 Mb target region). 136 patient DNA samples were collected for performance and application testing. Panel sensitivity and precision were measured using well-characterised DNA controls (n = 47), and specificity by Sanger sequencing of the Aryl Hydrocarbon Receptor Interacting Protein (AIP) gene in 89 patients. Assessment of liquid biopsy application employed a pool of synthetic circulating tumour DNA (ctDNA). Library preparation and sequencing were conducted on Illumina-based platforms prior to analysis with our accredited (ISO15189) bioinformatics pipeline. We achieved a mean coverage of 395x, with sensitivity and specificity of >99% and precision of >97%. Liquid biopsy revealed detection to 1.25% variant allele frequency. Application to head and neck tumours/cancers resulted in detection of mutations aligned to published databases. In conclusion, we have developed an analytically-validated panel for application to cancers of disparate types with utility in liquid biopsy. Next generation sequencing has revolutionised genomic studies of cancer, having facilitated the development of precision oncology treatments based on a tumour's molecular profile. We aimed to develop a targeted gene sequencing panel for application to disparate cancer types with particular focus on tumours of the head and neck, plus test for utility in liquid biopsy. The final panel designed through Roche/Nimblegen combined 451 cancer-associated genes (2.01 Mb target region). 136 patient DNA samples were collected for performance and application testing. Panel sensitivity and precision were measured using well-characterised DNA controls (n = 47), and specificity by Sanger sequencing of the Aryl Hydrocarbon Receptor Interacting Protein (AIP) gene in 89 patients. Assessment of liquid biopsy application employed a pool of synthetic circulating tumour DNA (ctDNA). Library preparation and sequencing were conducted on Illumina-based platforms prior to analysis with our accredited (ISO15189) bioinformatics pipeline. We achieved a mean coverage of 395x, with sensitivity and specificity of >99% and precision of >97%. Liquid biopsy revealed detection to 1.25% variant allele frequency. Application to head and neck tumours/cancers resulted in detection of mutations aligned to published databases. In conclusion, we have developed an analytically-validated panel for application to cancers of disparate types with utility in liquid biopsy.
ArticleNumber	17052
Author	Gupta, Ruta Dinger, Marcel E. Ying, Kevin Kohonen-Corish, Maija O’Toole, Sandra A. McCabe, Mark J. Cowley, Mark J. McCormack, Ann I. Swarbrick, Alex Thompson, Tanya J. Clark, Jonathan Mueller, Simon A. Gayevskiy, Velimir Chan, Chia-Ling De Sousa, Sunita M.C. Grady, John P. Deng, Niantao Cipponi, Arcadi Johns, Amber L. Thomas, Melissa L. Gauthier, Marie-Emilie A. Lord, Reginald V. Puttick, Clare Tao, Jiang
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givenname: Simon A. surname: Mueller fullname: Mueller, Simon A. organization: Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, The Sydney Head and Neck Cancer Institute, Chris O’Brien Lifehouse, Department for Oto-Rhino-Laryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern – sequence: 21 givenname: Ruta surname: Gupta fullname: Gupta, Ruta organization: The Sydney Head and Neck Cancer Institute, Chris O’Brien Lifehouse, Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred Hospital, Central Clinical School, The University of Sydney – sequence: 22 givenname: Ann I. surname: McCormack fullname: McCormack, Ann I. organization: Hormones and Cancer Group, Garvan Institute of Medical Research, St Vincent’s Clinical School, UNSW Australia, Department of Endocrinology, St Vincent’s Hospital – sequence: 23 givenname: Marcel E. surname: Dinger fullname: Dinger, Marcel E. organization: Kinghorn Centre for 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BackLink	https://www.ncbi.nlm.nih.gov/pubmed/31745186$$D View this record in MEDLINE/PubMed
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Cites_doi	10.1038/ejhg.2015.226 10.1016/S0140-6736(10)61121-X 10.1016/j.jaad.2017.08.059 10.1111/jocd.12378 10.1136/esmoopen-2016-000094 10.3389/fgene.2019.00049 10.1016/j.jmoldx.2017.01.011 10.1093/bioinformatics/bty540 10.3389/fonc.2019.00119 10.1371/journal.pcbi.1004873 10.1200/JCO.2015.66.2866 10.1016/j.jmoldx.2016.07.006 10.1038/gim.2017.119 10.1126/science.1099314 10.1038/s41598-017-02388-7 10.1126/scitranslmed.3007094 10.1200/PO.18.00183 10.1186/gm482 10.1371/journal.pone.0154038 10.1186/s13023-016-0516-x 10.5858/arpa.2016-0517-RA 10.1200/JCO.2010.31.7883 10.1056/NEJM200103153441101 10.1016/j.cell.2014.06.049 10.1038/nbt.2696 10.1210/jc.2006-1668 10.1056/NEJMoa070956 10.1038/s41467-018-06027-1 10.1371/journal.pcbi.1003153 10.1186/1758-3284-3-2 10.1016/S0140-6736(09)61196-X 10.1056/NEJMoa040938 10.1016/j.jmoldx.2016.08.007 10.1158/1078-0432.CCR-14-1768 10.3389/fcvm.2017.00011 10.1038/s41467-018-03215-x 10.1093/bib/bbs017 10.1038/sdata.2016.25 10.12688/wellcomeopenres.10069.1 10.1002/cncr.21898 10.3390/jpm8030030 10.1056/NEJMoa1213261 10.1038/s41598-017-00520-1 10.1186/s12943-018-0757-5 10.1200/JCO.2017.35.15_suppl.e23065 10.1056/NEJMoa062867 10.5858/arpa.2013-0625-OA 10.1530/EJE-16-0944 10.1016/j.cell.2016.12.015 10.1093/bioinformatics/btu549 10.1093/nar/gkw227 10.1101/mcs.a003764 10.1038/nbt.3520 10.1038/nature11003 10.1371/journal.pone.0140712 10.1186/gb-2011-12-11-r112 10.1038/srep39348 10.1002/0471250953.bi1110s43 10.1101/023754 10.18632/oncotarget.22116
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Copyright	The Author(s) 2019 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
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References	Weerts (CR40) 2017; 7 Van der Auwera (CR34) 2013; 43 O’Leary (CR28) 2018; 9 Zook (CR30) 2016; 3 Pickering (CR51) 2014; 20 Dawson (CR26) 2013; 368 Jennings (CR15) 2017; 19 CR38 Newman (CR58) 2014; 30 Chien (CR59) 2017; 35 McCabe (CR31) 2019; 5 Fowler (CR37) 2016; 1 Posner (CR45) 2007; 357 Castellanos (CR11) 2017; 7 De Sousa (CR29) 2017; 176 Talevich, Shain, Botton, Bastian (CR36) 2016; 12 Froyen (CR13) 2016; 11 Chou (CR24) 2013; 5 Daly (CR43) 2006; 91 Gayevskiy, Roscioli, Dinger, Cowley (CR33) 2018; 35 Lanman (CR56) 2015; 10 Celestino-Soper (CR17) 2017; 4 Suwinski (CR10) 2019; 10 Brue, Castinetti (CR44) 2016; 11 Lim, Lee, Diefenbach, Kefford, Rizos (CR25) 2018; 17 Yohe (CR16) 2015; 139 Lai (CR35) 2016; 44 Handler, Goldberg (CR52) 2018; 172 Paila, Chapman, Kirchner, Quinlan (CR32) 2013; 9 Bower (CR4) 2016; 34 Barretina (CR42) 2012; 483 Inman (CR53) 2018; 9 Veness, Palme, Morgan (CR49) 2006; 106 Madan, Lear, Szeimies (CR47) 2010; 375 Shu (CR54) 2017; 7 Zygogianni (CR46) 2011; 3 Mu, Lu, Chen, Li, Elliott (CR19) 2016; 18 Santani (CR18) 2017; 141 Minoche, Dohm, Himmelbauer (CR55) 2011; 12 Paez (CR6) 2004; 304 Hyman, Taylor, Baselga (CR2) 2017; 168 Morash, Mitchell, Beltran, Element, Pathak (CR21) 2018; 8 Que, Zwald, Schmults (CR48) 2018; 78 Patel (CR50) 2011; 29 Bettegowda (CR60) 2014; 6 Lynch (CR5) 2004; 350 Miller (CR9) 2017; 26 Hoadley (CR1) 2014; 158 Thorvalsdóttir, Robinson, Mesirov (CR39) 2013; 14 Newman (CR57) 2016; 34 Matthijs (CR14) 2016; 24 Slamon (CR7) 2001; 344 Freedman (CR8) 2018; 2 Lionel (CR12) 2018; 20 Wang, Bettegowda (CR27) 2017; 19 Bang (CR23) 2010; 376 Horak, Fröhling, Glimm (CR20) 2016; 1 Halperin (CR41) 2019; 9 Druker (CR3) 2006; 355 Frampton (CR22) 2013; 31 RB Lanman (52000_CR56) 2015; 10 W Mu (52000_CR19) 2016; 18 PBS Celestino-Soper (52000_CR17) 2017; 4 A Santani (52000_CR18) 2017; 141 A Fowler (52000_CR37) 2016; 1 GA Van der Auwera (52000_CR34) 2013; 43 J Barretina (52000_CR42) 2012; 483 DM Hyman (52000_CR2) 2017; 168 AF Daly (52000_CR43) 2006; 91 T Brue (52000_CR44) 2016; 11 C Bettegowda (52000_CR60) 2014; 6 H Bower (52000_CR4) 2016; 34 MJ Veness (52000_CR49) 2006; 106 AN Freedman (52000_CR8) 2018; 2 S Yohe (52000_CR16) 2015; 139 JM Zook (52000_CR30) 2016; 3 AM Newman (52000_CR58) 2014; 30 DJ Slamon (52000_CR7) 2001; 344 SMC De Sousa (52000_CR29) 2017; 176 CR Pickering (52000_CR51) 2014; 20 H Thorvalsdóttir (52000_CR39) 2013; 14 R Chien (52000_CR59) 2017; 35 AG Zygogianni (52000_CR46) 2011; 3 P Horak (52000_CR20) 2016; 1 A Chou (52000_CR24) 2013; 5 J Wang (52000_CR27) 2017; 19 AC Lionel (52000_CR12) 2018; 20 G Matthijs (52000_CR14) 2016; 24 GM Frampton (52000_CR22) 2013; 31 AM Newman (52000_CR57) 2016; 34 V Madan (52000_CR47) 2010; 375 GJ Inman (52000_CR53) 2018; 9 E Castellanos (52000_CR11) 2017; 7 E Talevich (52000_CR36) 2016; 12 MR Posner (52000_CR45) 2007; 357 G Froyen (52000_CR13) 2016; 11 TJ Lynch (52000_CR5) 2004; 350 P Suwinski (52000_CR10) 2019; 10 SY Lim (52000_CR25) 2018; 17 MZ Handler (52000_CR52) 2018; 172 JG Paez (52000_CR6) 2004; 304 MJA Weerts (52000_CR40) 2017; 7 U Paila (52000_CR32) 2013; 9 52000_CR38 SKT Que (52000_CR48) 2018; 78 EM Miller (52000_CR9) 2017; 26 BJ Druker (52000_CR3) 2006; 355 MJ McCabe (52000_CR31) 2019; 5 SJ Dawson (52000_CR26) 2013; 368 YJ Bang (52000_CR23) 2010; 376 B O’Leary (52000_CR28) 2018; 9 AE Minoche (52000_CR55) 2011; 12 KA Hoadley (52000_CR1) 2014; 158 Z Lai (52000_CR35) 2016; 44 V Gayevskiy (52000_CR33) 2018; 35 LJ Jennings (52000_CR15) 2017; 19 Y Shu (52000_CR54) 2017; 7 SC Patel (52000_CR50) 2011; 29 M Morash (52000_CR21) 2018; 8 RF Halperin (52000_CR41) 2019; 9
References_xml	– volume: 24 start-page: 2 year: 2016 end-page: 5 ident: CR14 article-title: Guidelines for diagnostic next-generation sequencing publication-title: Eur. J. Hum. Genet. doi: 10.1038/ejhg.2015.226 – volume: 376 start-page: 687 year: 2010 end-page: 607 ident: CR23 article-title: Trastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2-positive advanced gastric or gastro-oesophageal junction cancer (ToGA): a phase 3, open-label, randomized controlled trial publication-title: Lancet. doi: 10.1016/S0140-6736(10)61121-X – volume: 78 start-page: 237 year: 2018 end-page: 247 ident: CR48 article-title: Cutaneous squamous cell carcinoma: Incidence, risk factors, diagnosis, and staging publication-title: J. Am. Acad. Dermatol. doi: 10.1016/j.jaad.2017.08.059 – volume: 172 start-page: 232 year: 2018 end-page: 234 ident: CR52 article-title: Cutaneous squamous cell carcinoma of the scalp extending to the skull: a case report and review of the literature publication-title: J. Cosmet. Dermatol. doi: 10.1111/jocd.12378 – volume: 26 start-page: 102033 year: 2017 end-page: 102045 ident: CR9 article-title: Development and validation of a targeted next generation DNA sequencing panel outperforming whole exome sequencing for the identification of clinically relevant genetic variants publication-title: Oncotarget. – volume: 1 start-page: 1 year: 2016 end-page: 11 ident: CR20 article-title: Integrating next-generation sequencing into clinical oncology: strategies, promises and pitfalls publication-title: ESMO Open. doi: 10.1136/esmoopen-2016-000094 – volume: 10 start-page: 1 year: 2019 end-page: 16 ident: CR10 article-title: Advancing personalized medicine through the application of whole exome sequencing and big data analytics publication-title: Front. Genet. doi: 10.3389/fgene.2019.00049 – volume: 19 start-page: 341 year: 2017 end-page: 365 ident: CR15 article-title: Guidelines for validation of next-generation sequencing-based oncology panels: A joint consensus recommendation of the association for molecular pathology and college of American pathologists publication-title: J. Mol. Diagn. doi: 10.1016/j.jmoldx.2017.01.011 – volume: 35 start-page: 122 year: 2018 end-page: 125 ident: CR33 article-title: Seave: a comprehensive web platform for storing and interrogating human genomic variation publication-title: Bioinformatics. doi: 10.1093/bioinformatics/bty540 – volume: 9 start-page: 1 year: 2019 end-page: 19 ident: CR41 article-title: Leveraging spatial variation in tumor purity for improved somatic variant calling of archival tumor only samples publication-title: Front. Oncol. doi: 10.3389/fonc.2019.00119 – volume: 12 start-page: e1004873 year: 2016 end-page: e1004890 ident: CR36 article-title: CNVkit genome-wide copy number detection and visualization from targeted DNA sequencing publication-title: PLoS Comput. Biol. doi: 10.1371/journal.pcbi.1004873 – volume: 34 start-page: 2851 year: 2016 end-page: 2857 ident: CR4 article-title: Life expectancy of patients with chronic myeloid leukemia approaches the life expectancy of the general population publication-title: J. Clin. Oncol. doi: 10.1200/JCO.2015.66.2866 – volume: 18 start-page: 923 year: 2016 end-page: 932 ident: CR19 article-title: Sanger confirmation is required to achieve optimal sensitivity and specificity in next-generation panel testing publication-title: J. Mol. Diagn. doi: 10.1016/j.jmoldx.2016.07.006 – volume: 20 start-page: 435 year: 2018 end-page: 443 ident: CR12 article-title: Improved diagnostic yield compared with targeted gene sequencing panels suggests a role for whole-genome sequencing as a first-tier genetic test publication-title: Genet. Med. doi: 10.1038/gim.2017.119 – volume: 304 start-page: 1497 year: 2004 end-page: 1500 ident: CR6 article-title: EGFR mutations in lung cancer: correlation with clinical response to gefitinib therapy publication-title: Science. doi: 10.1126/science.1099314 – volume: 7 start-page: 2136 year: 2017 end-page: 2148 ident: CR40 article-title: Somatic tumor mutations detected by targeted next generation sequencing in minute amounts of serum-derived cell-free DNA publication-title: Sci. Rep. doi: 10.1038/s41598-017-02388-7 – volume: 6 start-page: 1 year: 2014 end-page: 25 ident: CR60 article-title: Detection of circulating tumor DNA in early- and late-stage human malignancies publication-title: Sci. Transl. Med. doi: 10.1126/scitranslmed.3007094 – volume: 2 start-page: 1 year: 2018 end-page: 13 ident: CR8 article-title: Use of next-generation sequencing tests to guide cancer treatment: results from a nationally representative survey of oncologists in the Unite States publication-title: JCO. Precis. Oncol. doi: 10.1200/PO.18.00183 – volume: 5 start-page: 78 year: 2013 end-page: 88 ident: CR24 article-title: Clinical and molecular characterization of amplified-pancreatic cancer publication-title: Genome Med. doi: 10.1186/gm482 – volume: 11 start-page: 1 year: 2016 end-page: 16 ident: CR13 article-title: Validation and application of a custom-designed targeted next-generation sequencing panel for the diagnostic mutational profiling of solid tumours publication-title: PLoS One. doi: 10.1371/journal.pone.0154038 – volume: 11 start-page: 135 year: 2016 end-page: 151 ident: CR44 article-title: The risks of overlooking the diagnosis of secreting pituitary adenomas publication-title: Orphanet J. Rare Dis. doi: 10.1186/s13023-016-0516-x – volume: 141 start-page: 787 year: 2017 end-page: 797 ident: CR18 article-title: Development and validation of targeted next-generation sequencing panels for detection of germline variants in inherited diseases publication-title: Arch. Pathol. Lab. Med. doi: 10.5858/arpa.2016-0517-RA – volume: 29 start-page: 1488 year: 2011 end-page: 1494 ident: CR50 article-title: Increasing incidence of oral tongue squamous cell carcinoma in young white women, age 18 to 44 years publication-title: J. Clin. Oncol. doi: 10.1200/JCO.2010.31.7883 – volume: 344 start-page: 783 year: 2001 end-page: 792 ident: CR7 article-title: Use of chemotherapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2 publication-title: N. Engl. J. Med. doi: 10.1056/NEJM200103153441101 – volume: 158 start-page: 929 year: 2014 end-page: 944 ident: CR1 article-title: Multi-platform analysis of 12 cancer types reveals molecular classification within and across tissues-of-origin publication-title: Cell. doi: 10.1016/j.cell.2014.06.049 – volume: 31 start-page: 1023 year: 2013 end-page: 1031 ident: CR22 article-title: Development and validation of a clinical cancer genomic profiling test based on massively parallel DNA sequencing publication-title: Nat. Biotechnol. doi: 10.1038/nbt.2696 – volume: 91 start-page: 4769 year: 2006 end-page: 4775 ident: CR43 article-title: High prevalence of pituitary adenomas: a cross-sectional study in the province of Liege, Belgium publication-title: J. Clin. Endocrinol. Metab. doi: 10.1210/jc.2006-1668 – volume: 357 start-page: 1705 year: 2007 end-page: 1715 ident: CR45 article-title: Cisplatin and Fluorouracil alone or with Docetaxel in head and neck cancer publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa070956 – volume: 9 start-page: 3667 year: 2018 end-page: 3680 ident: CR53 article-title: The genomic landscape of cutaneous SCC reveals drivers and a novel azathioprine associated with mutational signature publication-title: Nat. Commun. doi: 10.1038/s41467-018-06027-1 – volume: 9 start-page: e1003153 year: 2013 end-page: e1003160 ident: CR32 article-title: GEMINI: integrative exploration of genetic variation and genome annotations publication-title: PLoS Comput. Biol. doi: 10.1371/journal.pcbi.1003153 – volume: 3 start-page: 1 year: 2011 end-page: 2 ident: CR46 article-title: Oral squamous cell cancer: early detection and the role of alcohol and smoking publication-title: Head Neck Oncol. doi: 10.1186/1758-3284-3-2 – volume: 375 start-page: 673 year: 2010 end-page: 785 ident: CR47 article-title: Non-melanoma skin cancer publication-title: Lancet. doi: 10.1016/S0140-6736(09)61196-X – volume: 350 start-page: 2129 year: 2004 end-page: 2139 ident: CR5 article-title: Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa040938 – volume: 19 start-page: 24 year: 2017 end-page: 34 ident: CR27 article-title: Applications of DNA-based liquid biopsy for central nervous system neoplasms publication-title: J. Mol. Diagn. doi: 10.1016/j.jmoldx.2016.08.007 – volume: 20 start-page: 6582 year: 2014 end-page: 6592 ident: CR51 article-title: Mutational landscape of aggressive cutaneous squamous cell carcinoma publication-title: Clin. Cancer Res. doi: 10.1158/1078-0432.CCR-14-1768 – volume: 4 start-page: 1 year: 2017 end-page: 11 ident: CR17 article-title: Validation and utilization of a clinical next-generation sequencing panel for selected cardiovascular disorders publication-title: Front. Cardiovasc. Med. doi: 10.3389/fcvm.2017.00011 – volume: 9 start-page: 896 year: 2018 end-page: 905 ident: CR28 article-title: Early circulating tumor DNA dynamics and clonal selection with palbociclib and fulvestrant for breast cancer publication-title: Nat. Commun. doi: 10.1038/s41467-018-03215-x – volume: 14 start-page: 178 year: 2013 end-page: 192 ident: CR39 article-title: Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration publication-title: Brief. Bioinform. doi: 10.1093/bib/bbs017 – volume: 3 start-page: 1 year: 2016 end-page: 26 ident: CR30 article-title: Extensive sequencing of seven human genomes to characterize benchmark reference materials publication-title: Sci. Data. doi: 10.1038/sdata.2016.25 – volume: 1 start-page: 1 year: 2016 end-page: 12 ident: CR37 article-title: Accurate clinical detection of exon copy number variants in a targeted NGS panel using DECoN publication-title: Wellcome Open Res. doi: 10.12688/wellcomeopenres.10069.1 – volume: 106 start-page: 2389 year: 2006 end-page: 2396 ident: CR49 article-title: High-risk cutaneous squamous cell carcinoma of the head and neck: results from 266 treated patients with metastatic lymph node disease publication-title: Cancer. doi: 10.1002/cncr.21898 – volume: 8 start-page: 30 year: 2018 end-page: 38 ident: CR21 article-title: The role of next-generation sequencing in precision medicine: a review of outcomes in oncology publication-title: J. Pers. Med. doi: 10.3390/jpm8030030 – volume: 368 start-page: 1199 year: 2013 end-page: 1209 ident: CR26 article-title: Analysis of circulating tumor DNA to monitor breast cancer publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1213261 – volume: 7 start-page: 583 year: 2017 end-page: 593 ident: CR54 article-title: Circulating tumour DNA mutation profiling by targeted next generation sequencing provides guidance for personalized treatments in multiple cancer types publication-title: Sci. Rep. doi: 10.1038/s41598-017-00520-1 – volume: 17 start-page: 8 year: 2018 end-page: 21 ident: CR25 article-title: Liquid biomarkers in melanoma: detection and discovery publication-title: Mol. Cancer. doi: 10.1186/s12943-018-0757-5 – ident: CR38 – volume: 35 start-page: e23065 year: 2017 end-page: e23065 ident: CR59 article-title: Comprehensive detection of ctDNA variants at 0.1% allelic frequency using a broad targeted NGS panel for liquid biopsy research publication-title: J. Clin. Oncol. doi: 10.1200/JCO.2017.35.15_suppl.e23065 – volume: 355 start-page: 2408 year: 2006 end-page: 2417 ident: CR3 article-title: IRIS investigators five-year follow-up of patients receiving imatinib for chronic myeloid leaukemia publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa062867 – volume: 139 start-page: 204 year: 2015 end-page: 210 ident: CR16 article-title: Clinical validation of targeted next-generation sequencing for inherited disorders publication-title: Arch. Pathol. Lab. Med. doi: 10.5858/arpa.2013-0625-OA – volume: 176 start-page: 635 year: 2017 end-page: 644 ident: CR29 article-title: Germline variants in familial pituitary tumour syndrome genes are common in young patients and families with additional endocrine tumours publication-title: Eur. J. Endocrinol. doi: 10.1530/EJE-16-0944 – volume: 168 start-page: 584 year: 2017 end-page: 599 ident: CR2 article-title: Implementing genome-driven oncology publication-title: Cell. doi: 10.1016/j.cell.2016.12.015 – volume: 30 start-page: 3390 year: 2014 end-page: 3393 ident: CR58 article-title: FACTERA: a practical method for the discovery of genomic rearrangements at breakpoint resolution publication-title: Bioinformatics. doi: 10.1093/bioinformatics/btu549 – volume: 44 start-page: e108 year: 2016 end-page: e118 ident: CR35 article-title: VarDict: a novel and versatile variant caller for next-generation sequencing in cancer research publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkw227 – volume: 5 start-page: 1 year: 2019 end-page: 19 ident: CR31 article-title: Genomic stratification and liquid biopsy in a rare adrenocortical carcinoma (ACC) case, with dual lung metastases publication-title: Cold Spring Harb. Mol. Case. Stud. doi: 10.1101/mcs.a003764 – volume: 34 start-page: 547 year: 2016 end-page: 555 ident: CR57 article-title: Integrated digital error suppression for improved detection of circulating tumor DNA publication-title: Nat. Biotechnol. doi: 10.1038/nbt.3520 – volume: 483 start-page: 603 year: 2012 end-page: 607 ident: CR42 article-title: The Cancer Cell Line Encyclopedia enables predictive modeling of anticancer drug sensitivity publication-title: Nature. doi: 10.1038/nature11003 – volume: 10 start-page: e0140712 year: 2015 end-page: e0140738 ident: CR56 article-title: Analytical clinical validation of a digital sequencing panel for quantitative, highly accurate evaluation of cell-free circulating tumour DNA publication-title: PLoS One. doi: 10.1371/journal.pone.0140712 – volume: 43 start-page: 11.10.1 year: 2013 end-page: 11.10.33 ident: CR34 article-title: From FastQ data to high-confidence variant calls: the Genome Analysis Toolkit best practices pipeline publication-title: Curr. Protoc. Bioinformatics. – volume: 12 start-page: R112 year: 2011 end-page: R126 ident: CR55 article-title: Evaluation of genomic high-throughput sequencing data generated on Illumina HiSeq and genome analyzer systems publication-title: Genome Biol. doi: 10.1186/gb-2011-12-11-r112 – volume: 7 start-page: 1 year: 2017 end-page: 2 ident: CR11 article-title: A comprehensive custom panel design for routine hereditary cancer testing: preserving control, improving diagnostics and revealing a complex variation landscape publication-title: Sci. Rep. doi: 10.1038/srep39348 – volume: 35 start-page: e23065 year: 2017 ident: 52000_CR59 publication-title: J. Clin. Oncol. doi: 10.1200/JCO.2017.35.15_suppl.e23065 – volume: 29 start-page: 1488 year: 2011 ident: 52000_CR50 publication-title: J. Clin. Oncol. doi: 10.1200/JCO.2010.31.7883 – volume: 35 start-page: 122 year: 2018 ident: 52000_CR33 publication-title: Bioinformatics. doi: 10.1093/bioinformatics/bty540 – volume: 30 start-page: 3390 year: 2014 ident: 52000_CR58 publication-title: Bioinformatics. doi: 10.1093/bioinformatics/btu549 – volume: 43 start-page: 11.10.1 year: 2013 ident: 52000_CR34 publication-title: Curr. Protoc. Bioinformatics. doi: 10.1002/0471250953.bi1110s43 – volume: 44 start-page: e108 year: 2016 ident: 52000_CR35 publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkw227 – volume: 2 start-page: 1 year: 2018 ident: 52000_CR8 publication-title: JCO. Precis. Oncol. doi: 10.1200/PO.18.00183 – volume: 1 start-page: 1 year: 2016 ident: 52000_CR20 publication-title: ESMO Open. doi: 10.1136/esmoopen-2016-000094 – volume: 7 start-page: 583 year: 2017 ident: 52000_CR54 publication-title: Sci. Rep. doi: 10.1038/s41598-017-00520-1 – volume: 172 start-page: 232 year: 2018 ident: 52000_CR52 publication-title: J. Cosmet. Dermatol. doi: 10.1111/jocd.12378 – volume: 78 start-page: 237 year: 2018 ident: 52000_CR48 publication-title: J. Am. Acad. Dermatol. doi: 10.1016/j.jaad.2017.08.059 – volume: 355 start-page: 2408 year: 2006 ident: 52000_CR3 publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa062867 – volume: 304 start-page: 1497 year: 2004 ident: 52000_CR6 publication-title: Science. doi: 10.1126/science.1099314 – volume: 34 start-page: 2851 year: 2016 ident: 52000_CR4 publication-title: J. Clin. Oncol. doi: 10.1200/JCO.2015.66.2866 – volume: 8 start-page: 30 year: 2018 ident: 52000_CR21 publication-title: J. Pers. Med. doi: 10.3390/jpm8030030 – ident: 52000_CR38 doi: 10.1101/023754 – volume: 11 start-page: 135 year: 2016 ident: 52000_CR44 publication-title: Orphanet J. Rare Dis. doi: 10.1186/s13023-016-0516-x – volume: 14 start-page: 178 year: 2013 ident: 52000_CR39 publication-title: Brief. Bioinform. doi: 10.1093/bib/bbs017 – volume: 375 start-page: 673 year: 2010 ident: 52000_CR47 publication-title: Lancet. doi: 10.1016/S0140-6736(09)61196-X – volume: 376 start-page: 687 year: 2010 ident: 52000_CR23 publication-title: Lancet. doi: 10.1016/S0140-6736(10)61121-X – volume: 24 start-page: 2 year: 2016 ident: 52000_CR14 publication-title: Eur. J. Hum. Genet. doi: 10.1038/ejhg.2015.226 – volume: 7 start-page: 2136 year: 2017 ident: 52000_CR40 publication-title: Sci. Rep. doi: 10.1038/s41598-017-02388-7 – volume: 91 start-page: 4769 year: 2006 ident: 52000_CR43 publication-title: J. Clin. Endocrinol. Metab. doi: 10.1210/jc.2006-1668 – volume: 6 start-page: 1 year: 2014 ident: 52000_CR60 publication-title: Sci. Transl. Med. doi: 10.1126/scitranslmed.3007094 – volume: 20 start-page: 435 year: 2018 ident: 52000_CR12 publication-title: Genet. Med. doi: 10.1038/gim.2017.119 – volume: 350 start-page: 2129 year: 2004 ident: 52000_CR5 publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa040938 – volume: 106 start-page: 2389 year: 2006 ident: 52000_CR49 publication-title: Cancer. doi: 10.1002/cncr.21898 – volume: 26 start-page: 102033 year: 2017 ident: 52000_CR9 publication-title: Oncotarget. doi: 10.18632/oncotarget.22116 – volume: 10 start-page: 1 year: 2019 ident: 52000_CR10 publication-title: Front. Genet. doi: 10.3389/fgene.2019.00049 – volume: 141 start-page: 787 year: 2017 ident: 52000_CR18 publication-title: Arch. Pathol. Lab. Med. doi: 10.5858/arpa.2016-0517-RA – volume: 1 start-page: 1 year: 2016 ident: 52000_CR37 publication-title: Wellcome Open Res. doi: 10.12688/wellcomeopenres.10069.1 – volume: 5 start-page: 1 year: 2019 ident: 52000_CR31 publication-title: Cold Spring Harb. Mol. Case. Stud. doi: 10.1101/mcs.a003764 – volume: 12 start-page: e1004873 year: 2016 ident: 52000_CR36 publication-title: PLoS Comput. Biol. doi: 10.1371/journal.pcbi.1004873 – volume: 9 start-page: 3667 year: 2018 ident: 52000_CR53 publication-title: Nat. Commun. doi: 10.1038/s41467-018-06027-1 – volume: 357 start-page: 1705 year: 2007 ident: 52000_CR45 publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa070956 – volume: 19 start-page: 341 year: 2017 ident: 52000_CR15 publication-title: J. Mol. Diagn. doi: 10.1016/j.jmoldx.2017.01.011 – volume: 10 start-page: e0140712 year: 2015 ident: 52000_CR56 publication-title: PLoS One. doi: 10.1371/journal.pone.0140712 – volume: 9 start-page: 896 year: 2018 ident: 52000_CR28 publication-title: Nat. Commun. doi: 10.1038/s41467-018-03215-x – volume: 5 start-page: 78 year: 2013 ident: 52000_CR24 publication-title: Genome Med. doi: 10.1186/gm482 – volume: 483 start-page: 603 year: 2012 ident: 52000_CR42 publication-title: Nature. doi: 10.1038/nature11003 – volume: 158 start-page: 929 year: 2014 ident: 52000_CR1 publication-title: Cell. doi: 10.1016/j.cell.2014.06.049 – volume: 3 start-page: 1 year: 2016 ident: 52000_CR30 publication-title: Sci. Data. doi: 10.1038/sdata.2016.25 – volume: 4 start-page: 1 year: 2017 ident: 52000_CR17 publication-title: Front. Cardiovasc. Med. doi: 10.3389/fcvm.2017.00011 – volume: 7 start-page: 1 year: 2017 ident: 52000_CR11 publication-title: Sci. Rep. doi: 10.1038/srep39348 – volume: 18 start-page: 923 year: 2016 ident: 52000_CR19 publication-title: J. Mol. Diagn. doi: 10.1016/j.jmoldx.2016.07.006 – volume: 34 start-page: 547 year: 2016 ident: 52000_CR57 publication-title: Nat. Biotechnol. doi: 10.1038/nbt.3520 – volume: 20 start-page: 6582 year: 2014 ident: 52000_CR51 publication-title: Clin. Cancer Res. doi: 10.1158/1078-0432.CCR-14-1768 – volume: 31 start-page: 1023 year: 2013 ident: 52000_CR22 publication-title: Nat. Biotechnol. doi: 10.1038/nbt.2696 – volume: 9 start-page: e1003153 year: 2013 ident: 52000_CR32 publication-title: PLoS Comput. Biol. doi: 10.1371/journal.pcbi.1003153 – volume: 3 start-page: 1 year: 2011 ident: 52000_CR46 publication-title: Head Neck Oncol. doi: 10.1186/1758-3284-3-2 – volume: 11 start-page: 1 year: 2016 ident: 52000_CR13 publication-title: PLoS One. doi: 10.1371/journal.pone.0154038 – volume: 17 start-page: 8 year: 2018 ident: 52000_CR25 publication-title: Mol. Cancer. doi: 10.1186/s12943-018-0757-5 – volume: 12 start-page: R112 year: 2011 ident: 52000_CR55 publication-title: Genome Biol. doi: 10.1186/gb-2011-12-11-r112 – volume: 9 start-page: 1 year: 2019 ident: 52000_CR41 publication-title: Front. Oncol. doi: 10.3389/fonc.2019.00119 – volume: 344 start-page: 783 year: 2001 ident: 52000_CR7 publication-title: N. Engl. J. Med. doi: 10.1056/NEJM200103153441101 – volume: 368 start-page: 1199 year: 2013 ident: 52000_CR26 publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1213261 – volume: 19 start-page: 24 year: 2017 ident: 52000_CR27 publication-title: J. Mol. Diagn. doi: 10.1016/j.jmoldx.2016.08.007 – volume: 168 start-page: 584 year: 2017 ident: 52000_CR2 publication-title: Cell. doi: 10.1016/j.cell.2016.12.015 – volume: 139 start-page: 204 year: 2015 ident: 52000_CR16 publication-title: Arch. Pathol. Lab. Med. doi: 10.5858/arpa.2013-0625-OA – volume: 176 start-page: 635 year: 2017 ident: 52000_CR29 publication-title: Eur. J. Endocrinol. doi: 10.1530/EJE-16-0944
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Snippet	Next generation sequencing has revolutionised genomic studies of cancer, having facilitated the development of precision oncology treatments based on a...
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SubjectTerms	45/23 45/41 45/47 631/208/514/2254 631/67/69 692/4028/67/69 Bioinformatics Biomarkers, Tumor - genetics Biopsy Cancer Carcinoma, Pancreatic Ductal - genetics Cell Line, Tumor Circulating Tumor DNA - genetics Computational Biology - methods Deoxyribonucleic acid DNA DNA sequencing Gene frequency Genetic Predisposition to Disease - genetics Genetic testing Genomics - methods Head & neck cancer Head and Neck Neoplasms - genetics High-Throughput Nucleotide Sequencing - methods Humanities and Social Sciences Humans Liquid Biopsy multidisciplinary Next-generation sequencing Oncology Pancreatic Neoplasms - genetics Pituitary Neoplasms - genetics Precision medicine Precision Medicine - methods Science Science (multidisciplinary) Sensitivity and Specificity Squamous Cell Carcinoma of Head and Neck - genetics Tumors
Title	Development and validation of a targeted gene sequencing panel for application to disparate cancers
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