Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility

CRISPR–Cas9 genome engineering is a powerful technology for correcting genetic diseases. However, the targeting range of Cas9 proteins is limited by their requirement for a protospacer adjacent motif (PAM), and in vivo delivery is challenging due to their large size. Here, we use phage-assisted cont...

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Veröffentlicht in:	Nature chemical biology Jg. 20; H. 3; S. 333 - 343
Hauptverfasser:	Schmidheini, Lukas, Mathis, Nicolas, Marquart, Kim Fabiano, Rothgangl, Tanja, Kissling, Lucas, Böck, Desirée, Chanez, Christelle, Wang, Jingrui Priscilla, Jinek, Martin, Schwank, Gerald
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	New York Nature Publishing Group US 01.03.2024 Nature Publishing Group
Schlagworte:	631/154/152 631/92/469 Biochemical Engineering Biochemistry Biocompatibility Bioorganic Chemistry Cell Biology Chemistry Chemistry and Materials Science Chemistry/Food Science CRISPR CRISPR-Associated Protein 9 - genetics CRISPR-Associated Protein 9 - metabolism CRISPR-Cas Systems - genetics Directed evolution Editing Evolution Gene Editing Genetic disorders Genetic modification Genome Genome editing Genomes Mutation Nuclease Robustness
ISSN:	1552-4450, 1552-4469, 1552-4469
Online-Zugang:	Volltext
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