Ultra-high-frequency radio-frequency acoustic molecular imaging with saline nanodroplets in living subjects

Molecular imaging is a crucial technique in clinical diagnostics but it relies on radioactive tracers or strong magnetic fields that are unsuitable for many patients, particularly infants and pregnant women. Ultra-high-frequency radio-frequency acoustic (UHF-RF-acoustic) imaging using non-ionizing R...

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Vydáno v:	Nature nanotechnology Ročník 16; číslo 6; s. 717 - 724
Hlavní autoři:	Chen, Yun-Sheng, Zhao, Yang, Beinat, Corinne, Zlitni, Aimen, Hsu, En-Chi, Chen, Dong-Hua, Achterberg, Friso, Wang, Hanwei, Stoyanova, Tanya, Dionne, Jennifer, Gambhir, Sanjiv Sam
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England Nature Publishing Group 01.06.2021
Témata:	Acoustic imaging Acoustics Animal models Animals Biocompatibility Cell Line, Tumor Chemical synthesis Contrast agents Contrast media Contrast Media - chemistry Drug Stability Gastrin Humans Hydrocarbons, Fluorinated - chemistry Iron oxides Magnetic fields Male Medical imaging Mice Mice, Inbred NOD Molecular Imaging - instrumentation Molecular Imaging - methods Nanoparticles Nanostructures - chemistry Perfluorocarbons Phantoms, Imaging Prostate cancer Prostatic Neoplasms - diagnostic imaging Prostatic Neoplasms - metabolism Radio frequency Radio Waves Radioactive tracers Receptors Receptors, Bombesin - genetics Receptors, Bombesin - immunology Receptors, Bombesin - metabolism Saline Solution, Hypertonic - chemistry Saline solutions Spatial discrimination Spatial resolution Ultrahigh frequencies Xenograft Model Antitumor Assays Xenografts Xenotransplantation
ISSN:	1748-3387, 1748-3395, 1748-3395
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Shrnutí:	Molecular imaging is a crucial technique in clinical diagnostics but it relies on radioactive tracers or strong magnetic fields that are unsuitable for many patients, particularly infants and pregnant women. Ultra-high-frequency radio-frequency acoustic (UHF-RF-acoustic) imaging using non-ionizing RF pulses allows deep-tissue imaging with sub-millimetre spatial resolution. However, lack of biocompatible and targetable contrast agents has prevented the successful in vivo application of UHF-RF-acoustic imaging. Here we report our development of targetable nanodroplets for UHF-RF-acoustic molecular imaging of cancers. We synthesize all-liquid nanodroplets containing hypertonic saline that are stable for at least 2 weeks and can produce high-intensity UHF-RF-acoustic signals. Compared with concentration-matched iron oxide nanoparticles, our nanodroplets produce at least 1,600 times higher UHF-RF-acoustic signals at the same imaging depth. We demonstrate in vivo imaging using the targeted nanodroplets in a prostate cancer xenograft mouse model expressing gastrin release protein receptor (GRPR), and show that targeting specificity is increased by more than 2-fold compared with untargeted nanodroplets or prostate cancer cells not expressing this receptor.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1748-3387 1748-3395 1748-3395
DOI:	10.1038/s41565-021-00869-5