Up-regulation of CB1 cannabinoid receptors located at glutamatergic terminals in the medial prefrontal cortex of the obese Zucker rat

The present study describes a detailed neuroanatomical distribution map of the cannabinoid type 1 (CB 1 ) receptor, along with the biochemical characterization of the expression and functional coupling to their cognate G i/o proteins in the medial prefrontal cortex (mPCx) of the obese Zucker rats. T...

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Veröffentlicht in:Frontiers in neuroanatomy Jg. 16; S. 1004702
Hauptverfasser: Echeazarra, Leyre, Barrondo, Sergio, García del Caño, Gontzal, Bonilla-Del Río, Itziar, Egaña-Huguet, Jon, Puente, Nagore, Aretxabala, Xabier, Montaña, Mario, López de Jesús, Maider, González-Burguera, Imanol, Saumell-Esnaola, Miquel, Goicolea, María Aránzazu, Grandes, Pedro, Sallés, Joan
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Lausanne Frontiers Research Foundation 18.10.2022
Frontiers Media S.A
Schlagworte:
2-AG levels
Anatomy
Animals
Behavior
Brain
Cannabinoid CB1 receptors
Cannabinoids
CB1 receptor
Chromatography
Food
Genotype & phenotype
Gi/o proteins
glutamatergic terminals
Glutamatergic transmission
Hyperactivity
Laboratories
Light
Mass spectrometry
medial prefrontal cortex
Microscopy
Neocortex
Neuroanatomy
obese Zucker rat
Obesity
Prefrontal cortex
Receptor density
Rodents
Scientific imaging
Synaptic plasticity
Up-regulation
United States > US
Japan
Germany
Spain
ISSN:1662-5129, 1662-5129
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Zusammenfassung:The present study describes a detailed neuroanatomical distribution map of the cannabinoid type 1 (CB 1 ) receptor, along with the biochemical characterization of the expression and functional coupling to their cognate G i/o proteins in the medial prefrontal cortex (mPCx) of the obese Zucker rats. The CB 1 receptor density was higher in the prelimbic (PL) and infralimbic (IL) subregions of the mPCx of obese Zucker rats relative to their lean littermates which was associated with a higher percentage of CB 1 receptor immunopositive excitatory presynaptic terminals in PL and IL. Also, a higher expression of CB 1 receptors and WIN55,212-2-stimulated [ 35 S]GTPγS binding was observed in the mPCx but not in the neocortex (NCx) and hippocampus of obese rats. Low-frequency stimulation in layers II/III of the mPCx induced CB 1 receptor-dependent long-term synaptic plasticity in IL of area obese Zucker but not lean rats. Overall, the elevated 2-AG levels, up-regulation of CB 1 receptors, and increased agonist-stimulated [ 35 S]GTPγS binding strongly suggest that hyperactivity of the endocannabinoid signaling takes place at the glutamatergic terminals of the mPCx in the obese Zucker rat. These findings could endorse the importance of the CB 1 receptors located in the mPCx in the development of obesity in Zucker rats.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Edited by: Daniel A. Peterson, Rosalind Franklin University of Medicine and Science, United States
Reviewed by: Pankaj Gaur, Georgetown University, United States; Sandra Montagud Romero, University of Valencia, Spain
ISSN:1662-5129
1662-5129
DOI:10.3389/fnana.2022.1004702