Assessing the impact of an antigen-specific antibody response on atherosclerosis development in mice

The antibody immune response plays a critical role in atherosclerosis. Here, we present a protocol for assessing the impact of an antigen-specific germinal center antibody response on atherosclerosis development, using a pro-atherogenic mouse model deficient for the production of germinal-center-der...

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Published in:STAR protocols Vol. 4; no. 2; p. 102274
Main Authors: Del Monte-Monge, Alberto, Martos-Folgado, Inmaculada, Rodríguez-Ronchel, Ana, Ramiro, Almudena R.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 16.06.2023
Elsevier
Subjects:
Antibody
Flow Cytometry/Mass Cytometry
Immunology
Model Organisms
Protocol
Model Organisms
Immunology
Antibody
Flow Cytometry/Mass Cytometry
ISSN:2666-1667, 2666-1667
Online Access:Get full text
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Summary:The antibody immune response plays a critical role in atherosclerosis. Here, we present a protocol for assessing the impact of an antigen-specific germinal center antibody response on atherosclerosis development, using a pro-atherogenic mouse model deficient for the production of germinal-center-derived antibodies. We describe steps for bone marrow transfer from donor mice into irradiated recipient mice. We then detail immunization of mouse chimeras with atheroprotective malondialdehyde low-density lipoprotein during high-fat diet feeding and atherosclerosis burden analysis. For complete details on the use and execution of this protocol, please refer to Martos-Folgado et al. (2022).1 [Display omitted] •Detailed protocol to generate Ldlr−/−Prdm1fl/flAicda-Cre+/ki bone marrow chimeras•Detailed instructions of MDA-LDL immunization during atherosclerosis induction•Extraction and processing of mouse hearts for the study of atherosclerosis•Quantification of atherosclerosis in MDA-LDL-immunized Prdm1fl/flAicda-Cre+/ki mice Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The antibody immune response plays a critical role in atherosclerosis. Here, we present a protocol for assessing the impact of an antigen-specific germinal center antibody response on atherosclerosis development, using a pro-atherogenic mouse model deficient for the production of germinal-center-derived antibodies. We describe steps for bone marrow transfer from donor mice into irradiated recipient mice. We then detail immunization of mouse chimeras with atheroprotective malondialdehyde low-density lipoprotein during high-fat diet feeding and atherosclerosis burden analysis.
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ISSN:2666-1667
2666-1667
DOI:10.1016/j.xpro.2023.102274