Field investigation with real-time virus genetic characterisation support of a cluster of Ebola virus disease cases in Dubréka, Guinea, April to June 2015

On 11 May 2015, the Dubréka prefecture, Guinea, reported nine laboratory-confirmed cases of Ebola virus disease (EVD). None could be epidemiologically linked to cases previously reported in the prefecture. We describe the epidemiological and molecular investigations of this event. We used the Dubrék...

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Published in:Euro surveillance : bulletin européen sur les maladies transmissibles Vol. 23; no. 12; p. 20
Main Authors: Pini, Alessandro, Zomahoun, Delayo, Duraffour, Sophie, Derrough, Tarik, Charles, Myrna, Quick, Joshua, Loman, Nick, Cowley, Lauren, Leno, Mamadou, Ouedraogo, Nobila, Thiam, Oumou, Hernández-Romieu, Alfonso, Iko, Annie, Keita, Halimatou, Konate, Djiba, Soumah, Aboubacar Aboubak, Bouchouar, Etran, Ileka-Priouzeau, Samuel, Keita, Sakoba, Diallo, Boubacar, Cisse, Fode, Jansa, Josep, Carroll, Miles, Günther, Stephan, Severi, Ettore, Formenty, Pierre
Format: Journal Article
Language:English
Published: Sweden Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS) 22.03.2018
European Centre for Disease Prevention and Control (ECDC)
Subjects:
Contact Tracing
Disease Outbreaks - prevention & control
DNA, Viral - genetics
Ebola virus
Ebolavirus - genetics
Ebolavirus - isolation & purification
Epidemiology
Genomics
Guinea - epidemiology
Hemorrhagic Fever, Ebola - diagnosis
Hemorrhagic Fever, Ebola - epidemiology
Hemorrhagic Fever, Ebola - transmission
Hemorrhagic Fever, Ebola - virology
Humans
Phylogeny
Public health
Real time
Real-Time Polymerase Chain Reaction
Surveillance and Outbreak Report
Guinea
Guinea (country)
Ebola virus
viral haemorrhagic fever
laboratory surveillance
outbreaks
Guinea
ISSN:1560-7917, 1025-496X, 1560-7917
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Summary:On 11 May 2015, the Dubréka prefecture, Guinea, reported nine laboratory-confirmed cases of Ebola virus disease (EVD). None could be epidemiologically linked to cases previously reported in the prefecture. We describe the epidemiological and molecular investigations of this event. We used the Dubréka EVD registers and the Ebola treatment centre’s (ETC) records to characterise chains of transmission. Real-time field Ebola virus sequencing was employed to support epidemiological results. An epidemiological cluster of 32 cases was found, of which 27 were laboratory confirmed, 24 were isolated and 20 died. Real-time viral sequencing on 12 cases demonstrated SL3 lineage viruses with sequences differing by one to three nt inside a single phylogenetic cluster. For isolated cases, the average time between symptom onset and ETC referral was 2.8 days (interquartile range (IQR): 1–4). The average time between sample collection and molecular results’ availability was 3 days (IQR: 2–5). In an area with scarce resources, the genetic characterisation supported the outbreak investigations in real time, linking cases where epidemiological investigation was limited and reassuring that the responsible strain was already circulating in Guinea. We recommend coupling thorough epidemiological and genomic investigations to control EVD clusters.
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Correspondence: Ettore Severi (Ettore.severi@ecdc.europa.eu)
ISSN:1560-7917
1025-496X
1560-7917
DOI:10.2807/1560-7917.ES.2018.23.12.17-00140