The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)

The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	Genome research Ročník 14; číslo 10B; s. 2121
Hlavní autori:	Gerhard, Daniela S, Wagner, Lukas, Feingold, Elise A, Shenmen, Carolyn M, Grouse, Lynette H, Schuler, Greg, Klein, Steven L, Old, Susan, Rasooly, Rebekah, Good, Peter, Guyer, Mark, Peck, Allison M, Derge, Jeffery G, Lipman, David, Collins, Francis S, Jang, Wonhee, Sherry, Steven, Feolo, Mike, Misquitta, Leonie, Lee, Eduardo, Rotmistrovsky, Kirill, Greenhut, Susan F, Schaefer, Carl F, Buetow, Kenneth, Bonner, Tom I, Haussler, David, Kent, Jim, Kiekhaus, Mark, Furey, Terry, Brent, Michael, Prange, Christa, Schreiber, Kirsten, Shapiro, Nicole, Bhat, Narayan K, Hopkins, Ralph F, Hsie, Florence, Driscoll, Tom, Soares, M Bento, Casavant, Tom L, Scheetz, Todd E, Brown-stein, Michael J, Usdin, Ted B, Toshiyuki, Shiraki, Carninci, Piero, Piao, Yulan, Dudekula, Dawood B, Ko, Minoru S H, Kawakami, Koichi, Suzuki, Yutaka, Sugano, Sumio, Gruber, C E, Smith, M R, Simmons, Blake, Moore, Troy, Waterman, Richard, Johnson, Stephen L, Ruan, Yijun, Wei, Chia Lin, Mathavan, S, Gunaratne, Preethi H, Wu, Jiaqian, Garcia, Angela M, Hulyk, Stephen W, Fuh, Edwin, Yuan, Ye, Sneed, Anna, Kowis, Carla, Hodgson, Anne, Muzny, Donna M, McPherson, John, Gibbs, Richard A, Fahey, Jessica, Helton, Erin, Ketteman, Mark, Madan, Anuradha, Rodrigues, Stephanie, Sanchez, Amy, Whiting, Michelle, Madari, Anup, Young, Alice C, Wetherby, Keith D, Granite, Steven J, Kwong, Peggy N, Brinkley, Charles P, Pearson, Russell L, Bouffard, Gerard G, Blakesly, Robert W, Green, Eric D, Dickson, Mark C, Rodriguez, Alex C, Grimwood, Jane, Schmutz, Jeremy, Myers, Richard M, Butterfield, Yaron S N, Griffith, Malachi, Griffith, Obi L, Krzywinski, Martin I, Liao, Nancy, Morin, Ryan, Morrin, Ryan
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 01.10.2004
Predmet:	Animals Cloning, Molecular - methods Computational Biology DNA Primers DNA, Complementary - genetics DNA, Complementary - metabolism Gene Library Humans Mice National Institutes of Health (U.S.) Open Reading Frames - physiology Rats United States Xenopus laevis - genetics Zebrafish - genetics United States
ISSN:	1088-9051
On-line prístup:	Zistit podrobnosti o prístupe
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Popis
Shrnutí:	The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline.
Bibliografia:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1088-9051
DOI:	10.1101/gr.2596504