The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)

The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from...

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Veröffentlicht in:Genome research Jg. 14; H. 10B; S. 2121
Hauptverfasser: Gerhard, Daniela S, Wagner, Lukas, Feingold, Elise A, Shenmen, Carolyn M, Grouse, Lynette H, Schuler, Greg, Klein, Steven L, Old, Susan, Rasooly, Rebekah, Good, Peter, Guyer, Mark, Peck, Allison M, Derge, Jeffery G, Lipman, David, Collins, Francis S, Jang, Wonhee, Sherry, Steven, Feolo, Mike, Misquitta, Leonie, Lee, Eduardo, Rotmistrovsky, Kirill, Greenhut, Susan F, Schaefer, Carl F, Buetow, Kenneth, Bonner, Tom I, Haussler, David, Kent, Jim, Kiekhaus, Mark, Furey, Terry, Brent, Michael, Prange, Christa, Schreiber, Kirsten, Shapiro, Nicole, Bhat, Narayan K, Hopkins, Ralph F, Hsie, Florence, Driscoll, Tom, Soares, M Bento, Casavant, Tom L, Scheetz, Todd E, Brown-stein, Michael J, Usdin, Ted B, Toshiyuki, Shiraki, Carninci, Piero, Piao, Yulan, Dudekula, Dawood B, Ko, Minoru S H, Kawakami, Koichi, Suzuki, Yutaka, Sugano, Sumio, Gruber, C E, Smith, M R, Simmons, Blake, Moore, Troy, Waterman, Richard, Johnson, Stephen L, Ruan, Yijun, Wei, Chia Lin, Mathavan, S, Gunaratne, Preethi H, Wu, Jiaqian, Garcia, Angela M, Hulyk, Stephen W, Fuh, Edwin, Yuan, Ye, Sneed, Anna, Kowis, Carla, Hodgson, Anne, Muzny, Donna M, McPherson, John, Gibbs, Richard A, Fahey, Jessica, Helton, Erin, Ketteman, Mark, Madan, Anuradha, Rodrigues, Stephanie, Sanchez, Amy, Whiting, Michelle, Madari, Anup, Young, Alice C, Wetherby, Keith D, Granite, Steven J, Kwong, Peggy N, Brinkley, Charles P, Pearson, Russell L, Bouffard, Gerard G, Blakesly, Robert W, Green, Eric D, Dickson, Mark C, Rodriguez, Alex C, Grimwood, Jane, Schmutz, Jeremy, Myers, Richard M, Butterfield, Yaron S N, Griffith, Malachi, Griffith, Obi L, Krzywinski, Martin I, Liao, Nancy, Morin, Ryan, Morrin, Ryan
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.10.2004
Schlagworte:
Animals
Cloning, Molecular - methods
Computational Biology
DNA Primers
DNA, Complementary - genetics
DNA, Complementary - metabolism
Gene Library
Humans
Mice
National Institutes of Health (U.S.)
Open Reading Frames - physiology
Rats
United States
Xenopus laevis - genetics
Zebrafish - genetics
United States
ISSN:1088-9051
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Zusammenfassung:The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline.
Bibliographie:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1088-9051
DOI:10.1101/gr.2596504