A mouse model that is immunologically tolerant to reporter and modifier proteins

Reporter proteins have become an indispensable tool in biomedical research. However, exogenous introduction of these reporters into mice poses a risk of rejection by the immune system. Here, we describe the generation, validation and application of a multiple reporter protein tolerant ‘Tol' mou...

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Bibliographic Details
Published in:Communications biology Vol. 3; no. 1; p. 273
Main Authors: Bresser, Kaspar, Dijkgraaf, Feline E., Pritchard, Colin E. J., Huijbers, Ivo J., Song, Ji-Ying, Rohr, Jan C., Scheeren, Ferenc A., Schumacher, Ton N.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 29.05.2020
Nature Publishing Group
Subjects:
38/35
38/44
631/250/1619/554/1834
631/250/2152/569
631/61/17/1511
64/110
64/60
Biology
Biomedical and Life Sciences
CD8 antigen
Clonal deletion
Epitopes
Gene transfer
Immune system
Life Sciences
Lymphocytes T
Proteins
Rodents
ISSN:2399-3642, 2399-3642
Online Access:Get full text
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Summary:Reporter proteins have become an indispensable tool in biomedical research. However, exogenous introduction of these reporters into mice poses a risk of rejection by the immune system. Here, we describe the generation, validation and application of a multiple reporter protein tolerant ‘Tol' mouse model that constitutively expresses an assembly of shuffled reporter proteins from a single open reading frame. We demonstrate that expression of the Tol transgene results in the deletion of CD8 + T cells specific for a model epitope, and substantially improves engraftment of reporter-gene transduced T cells. The Tol strain provides a valuable mouse model for cell transfer and viral-mediated gene transfer studies, and serves as a methodological example for the generation of poly-tolerant mouse strains. Bresser and Dijkgraaf et al. develop the ‘Tol’ strain, a genetically modified mouse model that expresses a range of shuffled reporter and modifier proteins from a single open reading frame. This strain is immunologically tolerant to these reporter and modifier proteins, providing a valuable model system for cell transfer studies and virus-mediated gene transfer studies.
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ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-020-0979-0