Dual-Enzyme Cascade Composed of Chitosan Coated FeS2 Nanozyme and Glucose Oxidase for Sensitive Glucose Detection

Immobilizing enzymes with nanozymes to catalyze cascade reactions overcomes many of the shortcomings of biological enzymes in industrial manufacturing. In the study, glucose oxidases were covalently bound to FeS2 nanozymes as immobilization carriers while chitosan encapsulation increased the activit...

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Published in:Molecules (Basel, Switzerland) Vol. 28; no. 3; p. 1357
Main Authors: Shen, Bowen, Qing, Molan, Zhu, Liying, Wang, Yuxian, Jiang, Ling
Format: Journal Article
Language:English
Published: Basel MDPI AG 31.01.2023
MDPI
Subjects:
Chemical reactions
Composite materials
double-enzyme cascade
Efficiency
Enzymes
Glucose
glucose detection
immobilized enzyme
Morphology
Nanoparticles
nanozyme
Oxidation
Vibration
ISSN:1420-3049, 1420-3049
Online Access:Get full text
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Summary:Immobilizing enzymes with nanozymes to catalyze cascade reactions overcomes many of the shortcomings of biological enzymes in industrial manufacturing. In the study, glucose oxidases were covalently bound to FeS2 nanozymes as immobilization carriers while chitosan encapsulation increased the activity and stability of the immobilized enzymes. The immobilized enzymes exhibited a 10% greater increase in catalytic efficiency than the free enzymes while also being more stable and catalytically active in environments with an alkaline pH of 9.0 and a high temperature of 100 °C. Additionally, the FeS2 nanozyme-driven double-enzyme cascade reaction showed high glucose selectivity, even in the presence of lactose, dopamine, and uric acid, with a limit of detection (LOD) (S/N = 3) as low as 1.9 × 10−6 M. This research demonstrates that nanozymes may be employed as ideal carriers for biological enzymes and that the nanozymes can catalyze cascade reactions together with natural enzymes, offering new insights into interactions between natural and synthetic biosystems.
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ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28031357