Insights into ecological role of a new deltaproteobacterial order Candidatus Acidulodesulfobacterales by metagenomics and metatranscriptomics

Several abundant but yet uncultivated bacterial groups exist in extreme iron- and sulfur-rich environments, and the physiology, biodiversity, and ecological roles of these bacteria remain a mystery. Here we retrieved four metagenome-assembled genomes (MAGs) from an artificial acid mine drainage (AMD...

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Bibliographic Details
Published in:The ISME Journal Vol. 13; no. 8; pp. 2044 - 2057
Main Authors: Tan, Sha, Liu, Jun, Fang, Yun, Hedlund, Brian P., Lian, Zheng-Han, Huang, Li-Ying, Li, Jin-Tian, Huang, Li-Nan, Li, Wen-Jun, Jiang, Hong-Chen, Dong, Hai-Liang, Shu, Wen-Sheng
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01.08.2019
Oxford University Press
Subjects:
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Acid mine drainage
Adaptation, Physiological
Autotrophs
Biodiversity
Biomedical and Life Sciences
Candidatus Acidulodesulfobacterales
China
Deltaproteobacteria - genetics
Deltaproteobacteria - physiology
Drainage systems
Ecology
Environmental stress
Evolutionary Biology
Gene expression
Gene Expression Profiling
Genes
Genomes
Homology
Iron
Iron - metabolism
Life Sciences
Metabolic pathways
Metagenome - genetics
Metagenomics
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Microorganisms
Mining
Nitrogen Fixation
Nitrogenation
Oxidation
Oxidation-Reduction
Oxygen - metabolism
Reduction
Stress, Physiological
Sulfate reduction
Sulfides
Sulfides - chemistry
Sulfur
Sulfur - chemistry
Transcriptome
China
ISSN:1751-7362, 1751-7370, 1751-7370
Online Access:Get full text
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Summary:Several abundant but yet uncultivated bacterial groups exist in extreme iron- and sulfur-rich environments, and the physiology, biodiversity, and ecological roles of these bacteria remain a mystery. Here we retrieved four metagenome-assembled genomes (MAGs) from an artificial acid mine drainage (AMD) system, and propose they belong to a new deltaproteobacterial order, Candidatus Acidulodesulfobacterales. The distribution pattern of Ca . Acidulodesulfobacterales in AMDs across Southeast China correlated strongly with ferrous iron. Reconstructed metabolic pathways and gene expression profiles showed that they were likely facultatively anaerobic autotrophs capable of nitrogen fixation. In addition to dissimilatory sulfate reduction, encoded by dsrAB , dsrD , dsrL , and dsrEFH genes, these microorganisms might also oxidize sulfide, depending on oxygen concentration and/or oxidation reduction potential. Several genes with homology to those involved in iron metabolism were also identified, suggesting their potential role in iron cycling. In addition, the expression of abundant resistance genes revealed the mechanisms of adaptation and response to the extreme environmental stresses endured by these organisms in the AMD environment. These findings shed light on the distribution, diversity, and potential ecological role of the new order Ca . Acidulodesulfobacterales in nature.
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ISSN:1751-7362
1751-7370
1751-7370
DOI:10.1038/s41396-019-0415-y